RE: [Histonet] skeletal staining

From:"Rittman, Barry R"

No problem
Just make sure that you remove digestive tract as it tends to bind alizarin.


From: on behalf of Boyce, Amanda (NIH/NIAMS) [F]
Sent: Thu 1/26/2006 9:01 AM
Subject: [Histonet] skeletal staining

I'm in the middle of doing alcian blue/alizarin red whole skeletal chick preps and I have a question. The protocol I've chosen uses 95% ethanol for the fixative. I've already fixed in PFA.  Is it possible to continue with the protocol?  Are there any steps I should add?  Thanks, Amanda Boyce

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>