No problem
Just make sure that you remove digestive tract as it tends to bind alizarin.
Barry

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Boyce, Amanda (NIH/NIAMS) [F]
Sent: Thu 1/26/2006 9:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] skeletal staining



I'm in the middle of doing alcian blue/alizarin red whole skeletal chick preps and I have a question. The protocol I've chosen uses 95% ethanol for the fixative. I've already fixed in PFA.  Is it possible to continue with the protocol?  Are there any steps I should add?  Thanks, Amanda Boyce


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