Hi Sarka,

I do know that some chromogens, while advertised as permanent, can fade
completely in exposure to alcohol.  (When I was a Biotek Solutions rep, a
customer left his slides in alcohol while he went to lunch, and when he
returned, the color had completely faded.)  I don't know about this
particular chromogen, but it is something to keep in mind.  Do you always
quickly dehydrate and clear, or are there some days when the slides are in
alcohol longer than other days?

Bonnie Whitaker


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarka Lhotak
Sent: Tuesday, January 17, 2006 2:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Loosing NoveRed signal


Hello Histonetters,
this is a very annoying problem that I see intermitently. While developing a
colour with NovaRed, I can see a nice staining. I stop the reaction with
distilled water, do several changes in distilled and let sit for 10 minutes.
Then Hematoxylin, alcohols, xylenes and coverslipping in Permount. Once
counterstained and coverslipped, the staining is gone! THis problem is not
very frequent but all the more frustrating. Once I talked to Vector about
this and was advised to let the slides sit in water for 10 minutes before
hematoxylin. But it does not always help. Since the problem is intermittent
I still have not figured out what is happening. It is very frustrating, you
see a great staining but then it is gone and nobody will believe you! Has
anybody experienced this and have a remedy? Thanks for your help,

Sarka Lhotak

Henderson Research Centre, McMaster University,
Hamilton, Ontario

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