RE: [Histonet] Fixation time

From:"Charles Scouten"

The answer to this was posted in a lengthy article on the histonet about 2 years ago.  I saved it, it was very good, I would like to credit the author but forget the name.  Take a bow if you are reading this.  Meanwhile, you can search the archives but I will forward you an attachment of the article.

Charles W.  Scouten, Ph.D. 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 

-----Original Message-----
From: [] On Behalf Of Gudrun Lang
Sent: Tuesday, January 17, 2006 5:23 AM
To: Histonetliste (Histonetliste)
Subject: [Histonet] Fixation time

I have a question to all fixation-experts.
Can anyone tell me exact pentration- and fixation times, or the mm/hour for 4-8% NBF.
I know it depends on temperature, tissue-quality, but I have not found a table about this.

In "Histologische Technik, H.C. Burck" stands, that NBF takes 16 hours for 4mm in liver. (1mm/h) In the Microwave-book, Kok and Boon, stands NBF penetrates fast and the linkages develope slowly. But was is fast and slowly?

In daily work we let the tissue-blocks beetween 4-8 hours in NBF. Biopsies sometimes only 2 hours or less. 

Is the ratio 1mm/h really the rule of thumb for penetration and fixation?

Thank you

Gudrun Lang

Histolog. Labor
Akh Linz

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