RE: [Histonet] Counter stain

From:"Connolly, Brett M"


I've used 0.25% Metanil Yellow and/or Light Green...either one works fine,
just play with the times to get the staining intensity you want.


Brett M. Connolly, Ph.D.
Merck & Co., Inc.
MRL, Imaging Research
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-993-6803

-----Original Message-----
[] On Behalf Of
Sent: Thursday, January 19, 2006 1:29 PM
Subject: [Histonet] Counter stain

I am doing a peroxidase DAB stain for an antigen that is present only in
small quantities in the nucleus. I am doing it on rat bone. If I
counterstain with hematoxylin it covers the DAB stain. What else can I use
that will only stain the cytoplasm and not the nucleus. i thought of methyl
green but it too is supposed to stain the nucleus. I thought of neutral red
but it won't look so good with the brown. Suggestions?
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