RE: [Histonet] Counter stain - P.S.

From:"Monfils, Paul"

P.S.  Dyes like light green or fast green, which stain everything the same
shade and don't differentiate nuclei, are suitable as counterstains for some
immuno work.  However, in staining for a nuclear antigen it is particularly
important to have the nuclei visible.  Otherwise how can you be sure that
the staining you see is actually in the nuclei?  That's the advantage of
methyl green.  It doesn't stain anything very dark, but it does stain the
nuclei a little darker than the cytoplasm, so you can see where they are.

> ----------
> From: on behalf of
> Sent: 	Thursday, January 19, 2006 10:28 AM
> To:
> Subject: 	[Histonet] Counter stain
> I am doing a peroxidase DAB stain for an antigen that is present only in
> small quantities in the nucleus. I am doing it on rat bone. If I
> counterstain with hematoxylin it covers the DAB stain. What else can I use
> that will only stain the cytoplasm and not the nucleus. i thought of
> methyl green but it too is supposed to stain the nucleus. I thought of
> neutral red but it won't look so good with the brown. Suggestions?
> Cheers,
> Lorie
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