[Histonet] skeletal staining

From:"Boyce, Amanda \(NIH/NIAMS\) [F]"

I'm in the middle of doing alcian blue/alizarin red whole skeletal chick preps and I have a question. The protocol I've chosen uses 95% ethanol for the fixative. I've already fixed in PFA.  Is it possible to continue with the protocol?  Are there any steps I should add?  Thanks, Amanda Boyce

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