[Histonet] SUSPECT: RE: SUSPECT: Histonet Digest, Vol 26, Issue 31

From:"Tim Webster"

Yes Ma'am

 Tim Webster
Histology Specialist
Northwestern Medical Center
133 Fairfield Street
St Albans, VT 05478
(802) 524-1070 x4349 (Dept. & Voice mail)
twebster@nmcinc.org
 
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the author and not necessarily those of Norhtwestern Medical Center.
If you should receive this message in error, please delete it and notify the
sender. Thank you.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Thursday, January 26, 2006 11:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: SUSPECT: Histonet Digest, Vol 26, Issue 31

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Today's Topics:

   1. Hep Par 1 antibody (Martha Ward)
   2. Re: Zinc Formalin (John A. Kiernan)
   3. Re: (no subject) (John A. Kiernan)
   4. RE: Hep Par 1 antibody (GUTIERREZ, JUAN)
   5. RE: Hep Par 1 antibody (Sebree Linda A.)
   6. Re: Qu:Assesment of embryonic BBB defect (John A. Kiernan)
   7. RE: Helico Immuno (Cazares, Ruth)
   8. 	Wanted : Field Support Specialist - Histopathology, TEXAS
      (John Rynak)
   9. RE: Wanted : Field Support Specialist - Histopathology,	TEXAS
      (Malcolm McCallum)
  10. methacarn problem solved (kjsavage@buffalo.edu)
  11. seeking employment (pathrm35@adelphia.net)
  12. M2A (Patsy Ruegg)
  13. cytochrome c IHC or IF on mouse small intestine (Yu, Jian)
  14. Re: RE: GCK-like kinase(GLK) (Dana Marshall)
  15. Re: M2A (Gayle Callis)
  16. Sakura Tissue Tek II (Brusig, Stephanie)
  17. Positions available (Chaussey, Leslie)
  18. Texas Society for Histotechnology 2006 Program
      (KDwyer3322@aol.com)
  19. Quantitative Image Analysis Systems - -Any	recommendations?
      (Nicola Cragg)
  20. technique (Gimenez?) for rickettsiae (Bartlett, Jeanine)
  21. Re: technique (Gimenez?) for rickettsiae (Mildred Fail)
  22. RE: technique (Gimenez?) for rickettsiae (Bartlett, Jeanine)
  23. CORRECTION (Mildred Fail)
  24. Re: Quantitative Image Analysis Systems - -Any
      recommendations? (Kim Merriam)
  25. Re: technique (Gimenez?) for rickettsiae (Rene J Buesa)
  26. RE: technique (Gimenez?) for rickettsiae (Bartlett, Jeanine)
  27. skeletal staining (Boyce, Amanda (NIH/NIAMS) [F])
  28. Advice. (Ian Montgomery)
  29. Hep Par 1 antibody (Martha Ward)


----------------------------------------------------------------------

Message: 1
Date: Wed, 25 Jan 2006 13:22:22 -0500
From: "Martha Ward" 
Subject: [Histonet] Hep Par 1 antibody
To: 
Message-ID:
	
<61135F0455D33347B5AAE209B903A3041206E7D6@EXCHVS2.medctr.ad.wfubmc.edu>
	
Content-Type: text/plain;	charset="us-ascii"

I have approached by our Pathologists about working up the Hep Par 1
antibody and I was wondering if anyone out there is currently doing it,
and if so, could you recommend a vendor, conditions, etc.  Thanks for
much in advance for any help you can give me.
 
Martha Ward, MT (ASCP) QIHC
Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center


------------------------------

Message: 2
Date: Wed, 25 Jan 2006 13:26:22 -0500
From: "John A. Kiernan" 
Subject: Re: [Histonet] Zinc Formalin
To: Laurie Colbert 
Cc: "Histonet \(E-mail\)" 
Message-ID: <43D7C2CE.54157A1A@uwo.ca>
Content-Type: text/plain; charset=us-ascii

Zinc-formalin mixtures do not contain mercuric
chloride, so you will not need to do an iodine and
thiosulphate treatment of the sections. 

Ask your supplier to tell you the composition of
his "zinc formalin". Is it an acidic (zinc
sulphate or chloride) or a neutral (zinc
salicylate) formulation? Most zinc formalin
mixtures are acidic, and it may make a difference
if the anion is sulphate or chloride. The
published literature is clouded by trade secrecy,
as are the web sites of those who make the
mixtures. 

The acidic zinc formalin principle is simple:
combine a protein coagulant with a cross-linker.
All the classical late 19th century fixatives did
that, and more. Formalin pigment can be expected
if
tissues are stored for a long time in an acidic
formaldehyde solution.

Hope this helps.

John Kiernan
Anatomy, UWO
London, Canada.
______________________________________
Laurie Colbert wrote:
> 
> Our formalin supplier mistakenly shipped us pre-filled containers
containing zinc formalin instead of 10% NBF.  Before I noticed this, three
cases had been distributed throughout the hospital (most likely to Surgery).
So, how is this going to affect my tissue?  Is there any post-treatment (such
as removing mercury pigment on B-5 fixed tissue) that I need to perform?
> 
> Thanks in advance for any info you can provide.
> 
> Laurie Colbert
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 3
Date: Wed, 25 Jan 2006 13:29:06 -0500
From: "John A. Kiernan" 
Subject: Re: [Histonet] (no subject)
To: kjsavage@buffalo.edu
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <43D7C372.2EC99A99@uwo.ca>
Content-Type: text/plain; charset=us-ascii

Dear kjsavage@buffalo.edu 

Methacarn is easy to make. It contains nothing
that could form a precipitate. Your email
indicates that you have all the ingredients
(chloroform, methanol, acetic acid). This is a
non-aqueous coagulant fixative, differing from
Carnoy only in having methanol instead of ethanol.
Fixatives of this kind contain no water.

Cloudiness or a precipitate 
might happen if an ingredient is wrong or if 
water gets into the mixture. Are your methanol and
acetic acid 95-100%? 

The advice to avoid plastic was good, because
chloroform attacks some plastic ware. 

John Kiernan
Anatomy, UWO
London, Canada
______________________________________________________
kjsavage@buffalo.edu wrote:
> 
> Greetings all,
> 
> I am having a difficult time trying to make methacarn fixative.  I have
> searched the list archives and found a posting in May 2005 from someone
> that was having the same problem I am facing: a white precipitate
> forming.  In one of the responses to his posting it was suggested to
> use glass only (pipets, vials etc...).  I have done that, and still the
> precipitate forms.  I have also used all new reagents (chloroform,
> methanol, acetic acid) and still the precipitate forms.  Does anyone
> have some more suggestions as to what is going wrong?  Thanks, Kathy
> 
> Kathy Savage, PhD
> Dept. of Exercise and Nutrition Sciences
> SUNY-University at Buffalo
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 4
Date: Wed, 25 Jan 2006 12:34:30 -0600
From: "GUTIERREZ, JUAN" 
Subject: RE: [Histonet] Hep Par 1 antibody
To: "Martha Ward" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="US-ASCII"

We use DAKO's Hepatocyte antibody, clone OCH1E5.  It's very easy to work
up on the Ventana stainers. 

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer.  Long
live free speech!


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha
Ward
Sent: Wednesday, January 25, 2006 12:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hep Par 1 antibody

I have approached by our Pathologists about working up the Hep Par 1
antibody and I was wondering if anyone out there is currently doing it,
and if so, could you recommend a vendor, conditions, etc.  Thanks for
much in advance for any help you can give me.
 
Martha Ward, MT (ASCP) QIHC
Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 5
Date: Wed, 25 Jan 2006 12:46:21 -0600
From: "Sebree Linda A." 
Subject: RE: [Histonet] Hep Par 1 antibody
To: "Martha Ward" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="US-ASCII"

Martha,

We use Biocare's Hepatocyte Specific Antibody at 1:50 with a polymer DAB
kit on our Ventana automated immunostainers.  Our pathologists are happy
with it.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha
Ward
Sent: Wednesday, January 25, 2006 12:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hep Par 1 antibody


I have approached by our Pathologists about working up the Hep Par 1
antibody and I was wondering if anyone out there is currently doing it,
and if so, could you recommend a vendor, conditions, etc.  Thanks for
much in advance for any help you can give me.
 
Martha Ward, MT (ASCP) QIHC
Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 6
Date: Wed, 25 Jan 2006 13:48:10 -0500
From: "John A. Kiernan" 
Subject: Re: [Histonet] Qu:Assesment of embryonic BBB defect
To: Ali Moussavi Nik ,
	histonet@lists.utsouthwestern.edu
Message-ID: <43D7C7EA.CFA7A5D5@uwo.ca>
Content-Type: text/plain; charset=iso-8859-1

Immunostain for a plasma protein such as albumin.
It's important to fix the tissue quickly, or
plasma will move out of the blood vessels. See
Mori et al (1991) Lab. Invest. 64:345-351; Fabian
(1992) J. Histochem. Cytochem. 40: 987-991; Loberg
& Torvik (1992) Acta path. microbiol. scand. 100:
431-436. I've seen this artifact in human brain,
with immunostaining for albumin, ceruloplasmin,
orosomucoid and IgG (unpublished). The time of
first formation of the BBB is still controversial
(far too much literature to mention here) and may
depend on the techniques used.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Ali Moussavi Nik wrote:
> 
> Dear All
> 
> Hi
> 
> Dose any one knows how to asses the embryonic Blood Brain Barrier defect.
> 
> I would like to see whether or not the Blood Brain Barrier of 18.5 mouse
> embryo of a nock out strain is leaky.
> 
> I will extremely thankful if someone gives me a practical protocol.
> 
> 
> 
> 
> 
> 
> 
>
----------------------------------------------------------------------------
> ------
> 
> Seyed Ali Moussavi Nik D.V.M , PhD Student
> 
> Department of Cell and Molecular Biology,
> 
> Gothenburg University
> 
> Box 462
> 
> S-405 30 Göteborg
> 
> Sweden
> 
> Phone: +46(031) 7733895
> 
> Fax:     +46(031) 7733801
> 
> www.molbio.gu.se
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 7
Date: Wed, 25 Jan 2006 13:04:22 -0600
From: "Cazares, Ruth" 
Subject: [Histonet] RE: Helico Immuno
To: 
Message-ID:
	<913FAC2B773C19488E26AE6572180FA50458A5CF@exch01.schosp.org>
Content-Type: text/plain;	charset="us-ascii"

Thanks to all of you who responded to my inquiry on immunos for H.
pylori.

It really helps to get different opinions and perspectives to help one
weigh the pros and cons.

 

Sincerely,

Ruth Cazares

 



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Thank you for your cooperation.



------------------------------

Message: 8
Date: Wed, 25 Jan 2006 14:30:35 -0500
From: "John Rynak" 
Subject: [Histonet] 	Wanted : Field Support Specialist -
	Histopathology, TEXAS
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; format="flowed"


   Field Support Specialist - Histopathology

   SciGenium's  Client  is  experiencing  tremendous  growth within their
   North American operations. This growth is due to the 

   recent    launch   of   their   FDA   approved   automated   histology
   instrumentation system, and the growing antibody and reagent 

   consumable  product  line  for  the  histology  market. Due to the new
   launch of these products they are currently looking 

   for  Field  Support Specialist - Histopathology, for  Southwest Region
   (TX Houston or Dallas )

   
   Based  in Texas, this position provides technical applications support
   for our next generation range of automated histopathology products.
   This is a very hands on role where your key tasks will include:

   ˇ   Building an in-depth understanding of our new product technologies
   and their applications in a diagnostic histopathology environment; 

   ˇ   Customer  liaison by providing scientific and laboratory expertise
   for in-field installations, training and trouble-shooting; 

   ˇ  Customer support Help-line for remote problem solving; 

   ˇ  Designing and performing experiments to investigate and solve tough
   technical applications problems; and 

   ˇ   Preparing  problem  resolution reports and imparting your findings
   and  knowledge  to  internal  and  field  based  Product Engineers and
   Technical Managers.
   To be successful in this role you will possess:

   ˇ  Excellent problem solving and analytical skills 

   ˇ   The  ability to communicate with front-end users, technical design
   engineers and pragmatic sales force members. 

   ˇ   A tertiary qualification in a relevant science stream (eg. Medical
   Laboratory Science) 

   ˇ   Practical  experience  in  bench-level  histopathology,  including
   clinical immunohistochemistry 

   ˇ  Demonstrated use of automated scientific instrumentation
    BS/ MS in Life Sciences and ASCP Certification
   Interest  in  using  PC  based  software  and maintenance of automated
   scientific instrumentation will be highly advantageous.
   In addition, you are extremely detailed and analytically minded with a
   strong sense of customer focus. 

   Interested parties should forward a resume to resume@scigenium.com

   
   Send to :

      SciGenium

      PO 380916

      Cambridge, MA

      02238


------------------------------

Message: 9
Date: Wed, 25 Jan 2006 13:48:09 -0600
From: "Malcolm McCallum" 
Subject: RE: [Histonet] Wanted : Field Support Specialist -
	Histopathology,	TEXAS
To: "John Rynak" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

I am just wanting to clarify the employment situation for histotechs.
How hard is it to get vacancies filled?

Malcolm McCallum
A&M_Texarkana


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John
Rynak
Sent: Wednesday, January 25, 2006 1:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Wanted : Field Support Specialist - Histopathology,
TEXAS


   Field Support Specialist - Histopathology

   SciGenium's  Client  is  experiencing  tremendous  growth within
their
   North American operations. This growth is due to the 

   recent    launch   of   their   FDA   approved   automated
histology
   instrumentation system, and the growing antibody and reagent 

   consumable  product  line  for  the  histology  market. Due to the
new
   launch of these products they are currently looking 

   for  Field  Support Specialist - Histopathology, for  Southwest
Region
   (TX Houston or Dallas )

   
   Based  in Texas, this position provides technical applications
support
   for our next generation range of automated histopathology products.
   This is a very hands on role where your key tasks will include:

   *   Building an in-depth understanding of our new product
technologies
   and their applications in a diagnostic histopathology environment; 

   *   Customer  liaison by providing scientific and laboratory
expertise
   for in-field installations, training and trouble-shooting; 

   *  Customer support Help-line for remote problem solving; 

   *  Designing and performing experiments to investigate and solve
tough
   technical applications problems; and 

   *   Preparing  problem  resolution reports and imparting your
findings
   and  knowledge  to  internal  and  field  based  Product Engineers
and
   Technical Managers.
   To be successful in this role you will possess:

   *  Excellent problem solving and analytical skills 

   *   The  ability to communicate with front-end users, technical
design
   engineers and pragmatic sales force members. 

   *   A tertiary qualification in a relevant science stream (eg.
Medical
   Laboratory Science) 

   *   Practical  experience  in  bench-level  histopathology,
including
   clinical immunohistochemistry 

   *  Demonstrated use of automated scientific instrumentation
    BS/ MS in Life Sciences and ASCP Certification
   Interest  in  using  PC  based  software  and maintenance of
automated
   scientific instrumentation will be highly advantageous.
   In addition, you are extremely detailed and analytically minded with
a
   strong sense of customer focus. 

   Interested parties should forward a resume to resume@scigenium.com

   
   Send to :

      SciGenium

      PO 380916

      Cambridge, MA

      02238
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 10
Date: Wed, 25 Jan 2006 15:02:37 -0500
From: kjsavage@buffalo.edu
Subject: [Histonet] methacarn problem solved
To: histonet@lists.utsouthwestern.edu
Message-ID: <1138219357.43d7d95d8710a@mail3.buffalo.edu>
Content-Type: text/plain

Hi all,

First, "thank you" to everyone who responded to my please for help with 
the methacarn solution.  The problem was with the chloroform.  A 
chemist friend of mine clued me in to the problem.  

Most chloroform comes with ethanol added as a perservative.  But there 
are some with amylene as the preservative instead.  Sometimes they do 
not list it as an additive on the bottle, and you only find out when 
you call the company.  Filtering it out is an option.  But I guess now 
that I know what was happening I will just get the choloroform without 
amylene.

Thanks again to all for the helpful suggestions. 

Kathy Savage, PhD
Dept. of Exercise and Nutrition Sciences
SUNY-University at Buffalo



------------------------------

Message: 11
Date: Wed, 25 Jan 2006 15:34:14 -0500
From: pathrm35@adelphia.net
Subject: [Histonet] seeking employment
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<29876775.1138221254204.JavaMail.root@web12.mail.adelphia.net>
Content-Type: text/plain; charset=utf-8

 Histotechnologist seeking a lead tech or assistant supervisor position.
Willing to relocate and work any shift. I prefer private, high volume labs
and specialize in dermpath and IHC.
Thanks in advance.

Ron Martin, BS, HT (ASCP) HTL, QIHC



------------------------------

Message: 12
Date: Wed, 25 Jan 2006 14:40:20 -0700
From: "Patsy Ruegg" 
Subject: [Histonet] M2A
To: 
Message-ID: <200601252140.k0PLeJjH003601@chip.viawest.net>
Content-Type: text/plain;	charset="us-ascii"

has anyone used an antibody M2A Antigen (Lymphatic Endothelial Cell Marker)
for either swine or ovine tissue?  It works in human, but not canine, swine
or ovine.  I need swine or ovine.
Thanks,
Patsy
 
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg@ihctech.net
web site www.ihctech.net  
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

 


------------------------------

Message: 13
Date: Wed, 25 Jan 2006 16:55:44 -0500
From: "Yu, Jian" 
Subject: [Histonet] cytochrome c IHC or IF on mouse small intestine
To: 
Message-ID:
	<2554B4CA518D504A81E6908E39478A6A131FCD79@1upmc-msx10.isdip.upmc.edu>
Content-Type: text/plain;	charset="us-ascii"

Thanks a lot for the great suggestions I got from several of you on the
histopathology of mouse small intestine.  There are a lot published
results of cytochorme c release detected by IHC and IF in mouse liver
and brain, but hardly anything in small intestine.  Does anyone have a
good protocol for cytochrome c release IHC or IF in mouse small
intestine that you can share?  Or it does not work at all?

Thanks again!!!

 

********************************************************

Jian Yu, Ph. D.

Assistant Professor of Pathology

University of Pittsburgh Cancer Institute

Hillman Cancer Center Research Pavilion

Office suite 2.26h, Laboratory 2.43

5117 Centre Avenue, Pittsburgh, PA 15213

 

Phone : 412-623-7786, (Lab) 412-623-3255

Fax:      412-623-7778

Email:   yuj2@upmc.edu

********************************************************

 



------------------------------

Message: 14
Date: Wed, 25 Jan 2006 16:00:43 -0600
From: "Dana Marshall" 
Subject: Re: [Histonet] RE: GCK-like kinase(GLK)
To: "Edwards, R.E." ,
	
Message-ID: <005c01c621fa$c98e3ac0$f623c084@DanaM>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

http://www.genecards.org/cgi-bin/carddisp?MAP4K4&search=GCK-like+kinase&suff=
txt

this is a link to Weizman Institute's GeneCards (www.genecards.org)
i entered GCK-like kinase and as best i can tell it believes that is the 
same as map kinase kinase kinase kinase 4?  regardless, if that is not the 
right one, you might go ahead and try to find it on there using your own 
criteria.  as you scroll down the page you will see expression as reported 
using any number of methodologies as well as links out with more information 
about the expression data.  there is also a site called iHOP that has tons 
of information about proteins.
dana
----- Original Message ----- 
From: "Edwards, R.E." 
To: 
Sent: Wednesday, January 25, 2006 9:29 AM
Subject: [Histonet] RE: GCK-like kinase(GLK)






--Anyone  have  any  idea  of  the  distribution  of  GLK in  normal  human 
tissues, unable  to  find  anything  in  Pubmed.
                                                        Thanks
                                                           Richard  Edwards
                                                                MRC 
TOXICOLOGY UNIT
 
LEICESTER..U.K.....

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 15
Date: Wed, 25 Jan 2006 15:08:32 -0700
From: Gayle Callis 
Subject: Re: [Histonet] M2A
To: "Patsy Ruegg" ,
	Histonet@lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20060125150737.01b4a340@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Patsy,

Try this website, a new one for antibody location.

www.exactantigen.com

Good luck

At 02:40 PM 1/25/2006, you wrote:
>has anyone used an antibody M2A Antigen (Lymphatic Endothelial Cell Marker)

Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717




------------------------------

Message: 16
Date: Wed, 25 Jan 2006 14:18:32 -0800
From: "Brusig, Stephanie" 
Subject: [Histonet] Sakura Tissue Tek II
To: 
Message-ID:
	<16E971922EDF9A4B9E8D3216374EDD49766C30@wafedixm12.corp.weyer.pri>
Content-Type: text/plain;	charset="us-ascii"

Does any one have a operators manual they don't need for a Tissue Tek
II? I don't feel like paying the $125 from Sukura.
Thanks,

~Stephanie Brusig

Weyerhaeuser Company
Propagation of High Value Trees
WTC-1B10 
253.924.6518




------------------------------

Message: 17
Date: Wed, 25 Jan 2006 16:50:40 -0600
From: "Chaussey, Leslie" 
Subject: [Histonet] Positions available
To: 
Message-ID: 
Content-Type: text/plain;	charset="us-ascii"

Northwestern Memorial Hospital, consistently recognized as the most
preferred hospital in Chicago, is seeking new team members. We hire the
'Best People' who are dedicated to being a part of the 'Best Patient
Experience!' 

At Northwestern Memorial Hospital, we're setting new standards of care.
We're also creating career opportunities that allow you to share your
talent, develop new skills, and reach all of your goals. We're one of
the best hospitals in the world because we support the efforts, the
ideas, and the initiatives of all of our people. We invite you to join
us.

 

We are currently seeking qualified candidates for our Histology, Gross
Room, and Electron Microscopy/Renal Laboratories openings.  For more
information and to submit your resume, please visit our website,
www.nmh.org  .

 

AA/EOE

 




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------------------------------

Message: 18
Date: Wed, 25 Jan 2006 18:07:43 EST
From: KDwyer3322@aol.com
Subject: [Histonet] Texas Society for Histotechnology 2006 Program
To: histonet@lists.utsouthwestern.edu
Cc: , JWebb01@jpshealthnetwork.org, veronida@baylorhealth.edu
Message-ID: <1a0.44b03f7c.31095ebf@aol.com>
Content-Type: text/plain; charset="US-ASCII"

To all: 
The Texas Society for Histotechnology will be having it's 2006 
Symposium/Convention March 31-April 2, 2006 in Corpus Christi, Texas at the
Omni Hotel.  For 
a program or more information contact: 
Judy Webb - 817927-1024 or Veronica Davis -972-579-8291

Friday, March 31, 2006 10:00am-5:00 p.m.

NSH Sponsored Workshop
HT (ASCP) Examination Readiness -                                   Glenda 
Hoye BS, HT (ASCP)

                
Saturday, April 1, 2006  8-11:30 a.m.
A.M.  Workshops
#1    Reagent Alcohol-Can't Drink It-So What is It?                      Pam 
Marcum HT(ASCP)
#2    Technical Immunohistochemistry: Achieving Reliability and
     Reproducibility of Immunostains                                Rodney 
Miller, M.D.
#3    Thinking LEAN in Histology                                         R. 
Stephens HT(ASCP) QIHC
#4.   ASCP Certification Maintenance Program                             
Evelyn Sandberg, HT(ASCP)HTL

 
Saturday, April 1, 2006 1:00-4:30 p.m.
P.M.  Workshops   
#5    IHC Mathematics in the Laboratory                         Joel Martinez

/Fatima Natar
#6    MonKey Business-the Key to Delegation                      Jan Gardner 
,MBA,HT(ASCP)/
 

             Judi Stasko, BS, CLT
#7    ISH/FISH Theory and Application                             Noemi 
Sebastiao 
#8.   Gross Tissue Examination                                   Charles 
Embrey,BS,      
 

                      HT(ASCP), PA(ASCP)


Sunday,April 2, 2006   8:00-11:30 a.m.
AM Workshops  
#9 CSI: Corpus Christi, Case Study Investigations                        Mike

Reichenbach, HT (ASCP) QIHC

                                                Debra Flynn, HT(ASCP) QIHC
#10 Ready or Not Here It Comes: Microwave Technology        Donna Willis, 
HT(ASCP) HTL
#11   Do You Know What Your Laboratory Workflow Is?         Ritu Ward, 
MT(ASCP) MAOM 
#12.  Making Job Descriptions Work for You                          Jan 
Gardner, MBA, HT (ASCP)/
                                                                            
Judi Stasko, BS, CLT




------------------------------

Message: 19
Date: Thu, 26 Jan 2006 09:07:06 -0000
From: "Nicola Cragg" 
Subject: [Histonet] Quantitative Image Analysis Systems - -Any
	recommendations?
To: 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Hi All,

Has anyone got any recommendations and/or advice on image analysis
systems?  We are looking to buy a new image analysis system to quantify
IHC and perform histometric measurements (although this may require a
second system).  Does anyone have a system they can recommend?  Or has
anyone looked into this field and can offer any advice.  Up to now,
we've looked into Chromavision, Zeiss & Ariol.  

Thanks in advance,

Nicola Cragg
EpiStem Ltd.
Manchester, UK


------------------------------

Message: 20
Date: Thu, 26 Jan 2006 06:00:40 -0500
From: "Bartlett, Jeanine" 
Subject: [Histonet] technique (Gimenez?) for rickettsiae
To: 
Message-ID:
	
Content-Type: text/plain;	charset="US-ASCII"

Hi all:

Can anyone recommend a good technique for demonstration of rickettsiae
in FFPE tissue ?

Thanks,

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov



------------------------------

Message: 21
Date: Thu, 26 Jan 2006 07:30:30 -0500
From: "Mildred Fail" 
Subject: Re: [Histonet] technique (Gimenez?) for rickettsiae
To: , 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

Jeanine,
 Do both a Giemsa and Himenez, rickettsiae is + in both
Rena Fail

Rena Fail

>>> "Bartlett, Jeanine"  01/26/06 06:00AM >>>
Hi all:

Can anyone recommend a good technique for demonstration of rickettsiae
in FFPE tissue ?

Thanks,

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 22
Date: Thu, 26 Jan 2006 08:05:11 -0500
From: "Bartlett, Jeanine" 
Subject: RE: [Histonet] technique (Gimenez?) for rickettsiae
To: "Mildred Fail" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="US-ASCII"

They don't like the Giemsa for this:  they want something that will make
the bugs pop out at you. 


Jeanine Bartlett, HT(ASCP)
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: Mildred Fail [mailto:FAILM@musc.edu] 
Sent: Thursday, January 26, 2006 7:31 AM
To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] technique (Gimenez?) for rickettsiae

Jeanine,
 Do both a Giemsa and Himenez, rickettsiae is + in both Rena Fail

Rena Fail

>>> "Bartlett, Jeanine"  01/26/06 06:00AM >>>
Hi all:

Can anyone recommend a good technique for demonstration of rickettsiae
in FFPE tissue ?

Thanks,

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartlett@cdc.hhs.gov 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 23
Date: Thu, 26 Jan 2006 08:10:47 -0500
From: "Mildred Fail" 
Subject: [Histonet] CORRECTION
To: 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

gimenez and giemsa

Rena Fail




------------------------------

Message: 24
Date: Thu, 26 Jan 2006 05:45:31 -0800 (PST)
From: Kim Merriam 
Subject: Re: [Histonet] Quantitative Image Analysis Systems - -Any
	recommendations?
To: Nicola Cragg ,
	histonet@lists.utsouthwestern.edu
Message-ID: <20060126134531.15492.qmail@web50306.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

We have an Aperio system (http://www.aperio.com/), it scans the entire slide
at a 20X mag.  The resolution is absolutely amazing, we compared all of the
major brands mentioned below and this was by far the best system.  It also
comes with some nice image analysis software that can do pixel count,
nuclear, membrane and they can be modified to fit your specific needs.  We
have the T2 system that can scan 120 slides at a time.  It is definitely
worth looking into.
   
  Kim Merriam
  Novartis
  Cambridge, MA

Nicola Cragg  wrote:
  Hi All,

Has anyone got any recommendations and/or advice on image analysis
systems? We are looking to buy a new image analysis system to quantify
IHC and perform histometric measurements (although this may require a
second system). Does anyone have a system they can recommend? Or has
anyone looked into this field and can offer any advice. Up to now,
we've looked into Chromavision, Zeiss & Ariol. 

Thanks in advance,

Nicola Cragg
EpiStem Ltd.
Manchester, UK
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  



Kim Merriam
Novartis
Cambridge, MA
		
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------------------------------

Message: 25
Date: Thu, 26 Jan 2006 05:55:17 -0800 (PST)
From: Rene J Buesa 
Subject: Re: [Histonet] technique (Gimenez?) for rickettsiae
To: "Bartlett, Jeanine" ,
	histonet@lists.utsouthwestern.edu
Message-ID: <20060126135517.46518.qmail@web61219.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Jeanine:
  Between 1911 and 1945 there were 21 methods/variants developed for
rickettsiaeThe one by Wolbach (1919) stain nuclei and Rickettsiae blue and
cytoplasm red.
  Lépine's (1932) stain Rickettsiae red in pale brown cells. Many others are
variations of red agains blues (similar to Giemsa used for this purpose). I
prefer Wolbach's.
  Hope this will help.
  René J.

"Bartlett, Jeanine"  wrote:
  Hi all:

Can anyone recommend a good technique for demonstration of rickettsiae
in FFPE tissue ?

Thanks,

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA 30333
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  




		
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------------------------------

Message: 26
Date: Thu, 26 Jan 2006 09:03:51 -0500
From: "Bartlett, Jeanine" 
Subject: RE: [Histonet] technique (Gimenez?) for rickettsiae
To: "Rene J Buesa" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"

We use the Wolbach's Giemsa but he doesn't care for it for this purpose.
Thanks.
 

Jeanine Bartlett, HT(ASCP) 
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov 

 

  _____  

From: Rene J Buesa [mailto:rjbuesa@yahoo.com] 
Sent: Thursday, January 26, 2006 8:55 AM
To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] technique (Gimenez?) for rickettsiae


Jeanine:
Between 1911 and 1945 there were 21 methods/variants developed for
rickettsiaeThe one by Wolbach (1919) stain nuclei and Rickettsiae blue and
cytoplasm red.
Lépine's (1932) stain Rickettsiae red in pale brown cells. Many others are
variations of red agains blues (similar to Giemsa used for this purpose). I
prefer Wolbach's.
Hope this will help.
René J.

"Bartlett, Jeanine"  wrote:

	Hi all:
	
	Can anyone recommend a good technique for demonstration of
rickettsiae
	in FFPE tissue ?
	
	Thanks,
	
	Jeanine Bartlett, HT(ASCP)
	Centers for Disease Control and Prevention
	1600 Clifton Road, MS/G-32
	18/SB-114
	Atlanta, GA 30333
	(404) 639-3590 
	jeanine.bartlett@cdc.hhs.gov
	
	_______________________________________________
	Histonet mailing list
	Histonet@lists.utsouthwestern.edu
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
	




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------------------------------

Message: 27
Date: Thu, 26 Jan 2006 10:01:30 -0500
From: "Boyce, Amanda \(NIH/NIAMS\) [F]" 
Subject: [Histonet] skeletal staining
To: 
Message-ID:
	<72210BBE225D724FBDB0DF25C67B1A5305D99B@NIHCESMLBX9.nih.gov>
Content-Type: text/plain;	charset="iso-8859-1"

I'm in the middle of doing alcian blue/alizarin red whole skeletal chick
preps and I have a question. The protocol I've chosen uses 95% ethanol for
the fixative. I've already fixed in PFA.  Is it possible to continue with the
protocol?  Are there any steps I should add?  Thanks, Amanda Boyce




------------------------------

Message: 28
Date: Thu, 26 Jan 2006 15:29:33 +0000
From: Ian Montgomery 
Subject: [Histonet] Advice.
To: histonet@lists.utsouthwestern.edu
Message-ID: <7.0.1.0.2.20060126151836.03b48eb0@bio.gla.ac.uk>
Content-Type: text/plain; charset="us-ascii"; format=flowed

         With cuts in staff I've been asked if I can teach the 
histology portion of a zoology class. Problem, one of the tissue I'll 
be using is insect abdomen with its chitin exoskeleton. Any hints and 
tips regarding this type of material, processing, embedding, 
sectioning, that sort of thing. Or, is it Mollifix time again?

Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07623 975451
e-mail: ian.montgomery@bio.gla.ac.uk  




------------------------------

Message: 29
Date: Thu, 26 Jan 2006 10:39:52 -0500
From: "Martha Ward" 
Subject: [Histonet] Hep Par 1 antibody
To: 
Message-ID:
	
<61135F0455D33347B5AAE209B903A3041209894B@EXCHVS2.medctr.ad.wfubmc.edu>
	
Content-Type: text/plain;	charset="us-ascii"

I would like to thank everyone that responded to my question.  I hope to
get it up and running quickly.
 
Martha Ward
Wake Forest University Baptist Medical Center


------------------------------

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End of Histonet Digest, Vol 26, Issue 31
****************************************


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