[Histonet] Re: vertical lines in paraffin
What a coincedence! I had the same problem (years ago) when I worked for
Tulane Primate Research Center (now renamed) on the North Shore.
If you stain your slides and have no lines in your sections, it may be the
silicone coating they put on the slides at the factory. Sometimes it is
heavier than normal. Continue to use the same blade and after a couple of
blocks, the lines should go away.
If you continue to have problems, try stirring your paraffin before
embedding. The polymers may not be evenly distributed and will appear as
small white "floaters" in your holding tank.
Renee Grow, BA., HT (ASCP)
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN 37804-5016
(865) 977-5766 Fax
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