[Histonet] Re: H. Pylori work-up
Regarding H. Pylori:
It may be wise to use Steiner (instead of Giemsa) as a favored tissue-
based screening method for H. Pylori. In our experience, about 5-10%
of cases stained with Steiner end up being morphologically equivocal
where you view the organism but it doesn't have definitive
morphologic features of HP or HH. In those cases, we defer to IHC for
confirmation, which is the definitive tissue-based test. But I don't
think IHC should be run as a screening tool.
In terms of cost differences, a published article by Tulaymat et al.
in the Archives (I believe from 1999) actually shows that IHC is more
cost-effective. You could purchase a concentrated antibody and dilute
it out. In that case, the cost difference between silver stains an
IHC may not be that drastic.
Best,
Hadi Yaziji, M.D.
On Jan 24, 2006, at 1:10 PM, histonet-
request@lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. BrdU on fixed frozen section (Johnson, Teri)
> 2. BrdU Staining HCl pretreat (MVaughan4@ucok.edu)
> 3. sliding microtome vs rotary microtome (Malcolm McCallum)
> 4. How to get good cross sections of the mouse small intestine
> (Yu, Jian)
> 5. Embryons fixation and processing (ana.merino-trigo)
> 6. H.pylori immunos on gastric biopsies (Cazares, Ruth)
> 7. Human specific markers (Jim Manavis)
> 8. Re: Human specific markers (Jackie M O'Connor)
> 9. Re: H.pylori immunos on gastric biopsies (Rene J Buesa)
> 10. Re: How to get good cross sections of the mouse small
> intestine (Gayle Callis)
> 11. RE: H.pylori immunos on gastric biopsies (anita dudley)
> 12. RE: How to get good cross sections of the mouse small
> intestine (Monfils, Paul)
> 13. Re: Human specific markers (IGOR NASONKIN)
> 14. Source for "Citrusolve"... (Glenn Krasinski)
> 15. RE: Source for "Citrusolve"... (Bartlett, Jeanine)
> 16. Aw: BrdU Staining HCl pretreat (eva-moeller@arcor.de)
> 17. RE: H.pylori immunos on gastric biopsies (GUTIERREZ, JUAN)
> 18. CAP Question about Microwave (bsylinda@aol.com)
> 19. UN-SUBSCRIBE PLEASE (Tim Wheelock)
> 20. job opening (Slyter, Rodney)
> 21. (no subject) (kjsavage@buffalo.edu)
> 22. RE: H.pylori immunos on gastric biopsies (Bill Blank)
> 23. RE: How to get good cross sections of the mouse small
> intestine (Gayle Callis)
> 24. Re: CAP Question about Microwave (bsylinda@aol.com)
> 25. RE: H.pylori immunos on gastric biopsies
> (Smith, Jeffery D. (HSC))
> 26. Health Testing when using xylene (Sue Barnes)
> 27. Re: CAP Question about Microwave (Phil McArdle)
> 28. Re: Health Testing when using xylene (Rene J Buesa)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 23 Jan 2006 12:58:50 -0600
> From: "Johnson, Teri"
> Subject: [Histonet] BrdU on fixed frozen section
> To:
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID:
> institute.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Eva,
>
> Are you denaturing them in HCl prior to BrdU antibody? This antibody
> requires denaturing by HCl or microwaving in order to recognize the
> BrdU
> epitope. DNA in cultured cells and frozen sections should be denatured
> in 2-4M HCl treatment, rinsed, and then incubated in antibody.
>
> If you are currently doing this and still get no signal, send me your
> protocol (including dilutions) and we can go from there. (Direct
> detection vs. indirect detection) How long are your samples fixed
> prior
> to freezing?
>
> Teri Johnson, HT(ASCP)QIHC
> Managing Director Histology Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, MO 64133
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 23 Jan 2006 13:57:33 -0600
> From: MVaughan4@ucok.edu
> Subject: [Histonet] BrdU Staining HCl pretreat
> To: histonet@lists.utsouthwestern.edu
> Cc: eva-moeller@arcor.de
> Message-ID:
>
> Content-Type: text/plain; charset="ISO-8859-1"
>
> Eva,
> Did you pretreat the sample with 2N or 4N HCl?
>
> Mel
> Melville B. Vaughan, Ph. D.
> Assistant Professor
> Department of Biology
> University of Central Oklahoma
> 100 N. University Drive
> Edmond, OK 73034
>
> I am trying to do a BrdU staining on frozen tissue slices (already
> fixed
> with PFA 4%) for weeks.
> The signal is restricted to the rim of the core and not within it.
> I am
> staining with anti-BrdU by the Roche detection kit. I already tried
> their
> protocol but that did not work on my fixed slices.
> I dry the slices then rehydrate for 1 h. After that I apply the 1st
> antibody for 30 min 37°C.
> Thanks for support.
> Eva
>
> Machen Sie aus 14 Cent spielend bis zu 100 Euro!
> Die neue Gaming-Area von Arcor - über 50 Onlinespiele im Angebot.
> http://www.arcor.de/rd/emf-gaming-1
>
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 23 Jan 2006 14:06:01 -0600
> From: "Malcolm McCallum"
> Subject: [Histonet] sliding microtome vs rotary microtome
> To:
> Message-ID:
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi,
>
> I am planning some skeletochronology and we have a sliding
> microtome. I
> have always used a rotary microtome and am curious if there are any
> tips
> from the pros regarding its utility for this purpose.
>
>
>
> I haven't used a sliding microtome since I was an undergrad.
>
>
>
> Malcolm McCallum
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 23 Jan 2006 16:11:28 -0500
> From: "Yu, Jian"
> Subject: [Histonet] How to get good cross sections of the mouse small
> intestine
> To:
> Message-ID:
> <2554B4CA518D504A81E6908E39478A6A0D3CDF92@1upmc-msx10.isdip.upmc.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Dear all,
>
> I am a new histoneter. I am just starting to do some mouse small
> intestine histopathology and wondering whether anyone can provide a
> method (protocol) for how to fix and embed mouse small intestine to
> generate good cross (paraffin) sections with as many intact villi and
> crypts possible for H&E and IHC Particularly, I need to evaluate at
> least 5-10 different area of the small intestine to score the
> regeneration of crypts and the extend of apoptosis at various time
> point
> after irradiation.
>
> I have used neutral buffered formalin as fixative and manually bundle
> four pieces of 0.5 cm-long tubes together before embedding at a
> clinical
> pathology lab. The sections looked OK, but many appear to have a
> lot of
> floating villi in the lumen and limited number of crypts extending
> into
> to the villi connected to them. Since we can not always get our
> tissues processed after O/N fixation, we sometimes keep the tissues in
> NBF for several weeks. Is this too long, or should we transfer
> them to
> 70% ethanol?
>
>
>
> Look forward to your expert suggestions.
>
> With many thanks,
>
> ********************************************************
>
> Jian Yu, Ph. D.
>
> University of Pittsburgh Cancer Institute
>
> Hillman Cancer Center Research Pavilion
>
> Office suite 2.26h, Laboratory 2.43
>
> 5117 Centre Avenue, Pittsburgh, PA 15213
>
>
>
> Phone : 412-623-7786, (Lab) 412-623-3255
>
> Fax: 412-623-7778
>
> Email: yuj2@upmc.edu
>
> ********************************************************
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 23 Jan 2006 13:20:03 -0800
> From: "ana.merino-trigo"
> Subject: [Histonet] Embryons fixation and processing
> To: "Histonet"
> Message-ID: <031e01c62062$c6872fb0$5b790a0a@BABA>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello everybody,
>
> I just began a new job in a different field. Before I was working
> with mouse xenograft tumors and now I moved to develpment biology
> field. I need to process zebrafish, chick, axolt and mouse embryos
> and I was wondering if anyone could advice in terms of fixation and
> processing times. Mostly of the papers that I got they fix in 4%PFA
> (I'm use to fix in 10% formalin, and I was wondering if is common
> with embryos to fix with 4%PFA). In some papers they fix 2 h at RT
> or 4ºC o/n, when using 4%PFA. Any advice in this sense?
>
> In terms of processing, a guy that did some immunos with embryos
> here deshidrates samples in MeOH (25%, 50%, 75% and 100%, 5 min
> each), but I'm use to EtOH deshydratation (1x1h 70%; 2x1h 95%; 4x1
> h 100%). Could someone advice in deshydratation times and
> advantages of using MeOH?
>
> Thanks a lot,
>
> Ana
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 23 Jan 2006 15:34:27 -0600
> From: "Cazares, Ruth"
> Subject: [Histonet] H.pylori immunos on gastric biopsies
> To:
> Message-ID:
> <913FAC2B773C19488E26AE6572180FA50458A195@exch01.schosp.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Histonetters,
>
>
>
> I was wondering if anyone out there is doing immuno staining routinely
> on all gastric bx's? We are considering this in my lab, but I have my
> reservations. If some of you do, how many a week? And those who
> don't,
> why not? I will be doing a literature search on this topic, but if
> anyone knows of a specific article I sure would appreciate it if you
> could share it with me.
>
>
>
> Thank you,
>
>
>
> Ruth Cazares
>
>
>
>
>
> *** Confidentiality Statement ***
> This e-mail is intended only for the use of the individual or
> entity to which it is addressed and may contain information that is
> privileged and confidential. If the reader of this message is not
> the intended recipient, please notify the sender immediately by
> replying to this message and then delete it from your system. Any
> review, dissemination, distribution, or reproduction of this
> message by unintended recipients is strictly prohibited and may be
> subject to legal restriction.
>
>
> Thank you for your cooperation.
>
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 24 Jan 2006 08:23:42 +1030
> From: "Jim Manavis"
> Subject: [Histonet] Human specific markers
> To: "Histonet"
> Message-ID: <000701c62067$7a1dc710$011e0a0a@itp36533>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Can anyone suggest a human specific marker for use in
> Immnohistochemistry to
> distinguish between human and mouse cells
>
> Jim
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Mon, 23 Jan 2006 16:03:02 -0600
> From: "Jackie M O'Connor"
> Subject: Re: [Histonet] Human specific markers
> To: "Jim Manavis"
> Cc: Histonet
> Message-ID:
> Content-Type: text/plain; charset="us-ascii"
>
> Human mitochondria marker. I use it all the time.
>
>
>
>
>
>
> "Jim Manavis"
> Sent by: histonet-bounces@lists.utsouthwestern.edu
> 01/23/2006 03:53 PM
>
>
> To: "Histonet"
> cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT)
> Subject: [Histonet] Human specific markers
>
>
> Can anyone suggest a human specific marker for use in
> Immnohistochemistry
> to
> distinguish between human and mouse cells
>
> Jim
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Mon, 23 Jan 2006 13:55:22 -0800 (PST)
> From: Rene J Buesa
> Subject: Re: [Histonet] H.pylori immunos on gastric biopsies
> To: "Cazares, Ruth" ,
> histonet@pathology.swmed.edu
> Message-ID: <20060123215522.50894.qmail@web61215.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Ruth:
> I always used a phosphotungstic modification of the Steiner
> procedure to avoid
> the radioactive uranyl nitrate [J.Histotechnology, 24(2):113-6;
> 2001)
> I never considered doing IHC because of costs.
> The Steiner procedure cost was $0.91/slide and any "typical" IHC
> procedure
> would cost about $8/slide if done manually and about $6/slide if
> done automatic
> at low capacity.
> For me those were good reasons not to use IHC for H. pilorii.
> Hope this will help you.
> René J.
>
> "Cazares, Ruth" wrote:
> Hello Histonetters,
>
>
>
> I was wondering if anyone out there is doing immuno staining routinely
> on all gastric bx's? We are considering this in my lab, but I have my
> reservations. If some of you do, how many a week? And those who don't,
> why not? I will be doing a literature search on this topic, but if
> anyone knows of a specific article I sure would appreciate it if you
> could share it with me.
>
>
>
> Thank you,
>
>
>
> Ruth Cazares
>
>
>
>
>
> *** Confidentiality Statement ***
> This e-mail is intended only for the use of the individual or
> entity to which it is addressed and may contain information that is
> privileged and confidential. If the reader of this message is not
> the intended recipient, please notify the sender immediately by
> replying to this message and then delete it from your system. Any
> review, dissemination, distribution, or reproduction of this
> message by unintended recipients is strictly prohibited and may be
> subject to legal restriction.
>
>
> Thank you for your cooperation.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ---------------------------------
> Yahoo! Autos. Looking for a sweet ride? Get pricing, reviews, &
> more on new and used cars.
>
> ------------------------------
>
> Message: 10
> Date: Mon, 23 Jan 2006 15:59:24 -0700
> From: Gayle Callis
> Subject: [Histonet] Re: How to get good cross sections of the mouse
> small intestine
> To: "Yu, Jian" , Histonet@lists.utsouthwestern.edu
> Message-ID:
> <6.0.0.22.1.20060123154522.01b2cec0@gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Jian,
>
> You need to rinse the feces out of the lumen. Remove the small
> intestine
> INTACT but carefully by lifting it out of mouse. You can detach the
> mesentary as you do this but do not rip the sample out, you do NOT
> want to
> tear holes in the intestine. Fill a 50 ml syringe with PBS, and
> use a
> dulled 18 guage needle - insert into one end of the intestine
> (easier from
> the ilium side) and inject the PBS. After rinsing out feces, use a
> syringe
> filled with neutral buffered formalin, and inject that so the lumen
> distends. This will fix the villi much faster and get rid of
> digestive
> enzymes and left over fecal matter.
>
> You can clamp off the ends of the intestine or have sutures ready
> to tie
> off ends but you need to do this as the fixative is flowing out the
> opposite end of the intestine - overdistending the gut may not be a
> good
> idea, you need just enough to fix but not create a huge sausage like
> structure. Someone helping is nice, as they can tie off one end
> while the
> needle is removed to tie off the other end or use mosquito
> hemostats and
> clamp off. The idea is to fix the inside of intestine quickly to
> preserve
> villi. The gut can be stretched out in distended state or you can
> drop it
> into fixative then cut cross sections from each individual area of
> interest, process and embed on end. WE do this but NOT for cross
> sections,
> but mid sagittal sections however the intestine is well fixed, and
> with
> clean results.
>
> After fixation, you can store in 70% alcohol until processing.
> Just make
> sure you have total fixation or the 70% will finish it off for
> you. We are
> careful to not over process these tissues, they are small and
> delicate, and
> use a shorter processing schedule than for larger tissues.
>
> At 02:11 PM 1/23/2006, you wrote:
>> Dear all,
>>
>> I am a new histoneter. I am just starting to do some mouse small
>> intestine histopathology and wondering whether anyone can provide a
>> method (protocol) for how to fix and embed mouse small intestine to
>> generate good cross (paraffin) sections with as many intact villi and
>> crypts possible for H&E and IHC Particularly, I need to evaluate at
>> least 5-10 different area of the small intestine to score the
>> regeneration of crypts and the extend of apoptosis at various time
>> point
>> after irradiation.
>>
>> I have used neutral buffered formalin as fixative and manually bundle
>> four pieces of 0.5 cm-long tubes together before embedding at a
>> clinical
>> pathology lab. The sections looked OK, but many appear to have a
>> lot of
>> floating villi in the lumen and limited number of crypts extending
>> into
>> to the villi connected to them. Since we can not always get our
>> tissues processed after O/N fixation, we sometimes keep the
>> tissues in
>> NBF for several weeks. Is this too long, or should we transfer
>> them to
>> 70% ethanol?
>
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Mon, 23 Jan 2006 17:22:21 -0600
> From: "anita dudley"
> Subject: RE: [Histonet] H.pylori immunos on gastric biopsies
> To: RCazares@schosp.org, histonet@pathology.swmed.edu
> Message-ID:
> Content-Type: text/plain; format=flowed
>
> ruth, we do immuno on all of our gastric bx's. we do them every
> day, the
> tech that comes in early cuts them and puts them in the 58 oven.
> the drs
> like them coming to them with the case. I was in a lecture at nat.
> meeting
> and the person said that the immuno stain was one of the best ways to
> demonstrate them. I could probably find the lecture for you in my
> national
> notebooks, if you like.
> anita dudley
> providence hosp
> mobile alabama
>
>
>> From: "Cazares, Ruth"
>> To:
>> Subject: [Histonet] H.pylori immunos on gastric biopsies
>> Date: Mon, 23 Jan 2006 15:34:27 -0600
>>
>> Hello Histonetters,
>>
>>
>>
>> I was wondering if anyone out there is doing immuno staining
>> routinely
>> on all gastric bx's? We are considering this in my lab, but I
>> have my
>> reservations. If some of you do, how many a week? And those who
>> don't,
>> why not? I will be doing a literature search on this topic, but if
>> anyone knows of a specific article I sure would appreciate it if you
>> could share it with me.
>>
>>
>>
>> Thank you,
>>
>>
>>
>> Ruth Cazares
>>
>>
>>
>>
>>
>> *** Confidentiality Statement ***
>> This e-mail is intended only for the use of the individual or
>> entity to
>> which it is addressed and may contain information that is
>> privileged and
>> confidential. If the reader of this message is not the intended
>> recipient,
>> please notify the sender immediately by replying to this message
>> and then
>> delete it from your system. Any review, dissemination,
>> distribution, or
>> reproduction of this message by unintended recipients is strictly
>> prohibited and may be subject to legal restriction.
>>
>>
>> Thank you for your cooperation.
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Mon, 23 Jan 2006 18:44:44 -0500
> From: "Monfils, Paul"
> Subject: RE: [Histonet] How to get good cross sections of the mouse
> small intestine
> To: "'histonet@lists.utsouthwestern.edu'"
>
> Message-ID:
>
> <09C945920A6B654199F7A58A1D7D1FDE01717654@lsexch.lsmaster.lifespan.org
> >
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> In addition to Gayle's suggestions, you might want to physically
> hold the intestine straight while the formalin fixes and hardens
> it. After
> injecting formalin as Gayle said, I pin one end of it in a wax-lined
> dissection pan, then stretch it gently, just enough to straighten
> it, and
> pin the other end to keep it straight, then cover it with formalin.
> People
> sometimes send me intestine specimens which they have simply
> removed from
> the animal and dropped into formalin, resulting in a hardened
> gordian knot,
> all parts of which are curved to some degree, so that pieces cut
> from the
> intestine are shaped like parentheses. It's difficult to get a good
> perpendicular section of a curved object. If you don't have a
> dissecting
> pan you can easily make one; or you can just pin the specimen onto
> a strip
> of heavy corrugated cardbord or soft wood and drop it specimen side
> down
> into a dish of formalin.
>
>
>
> ------------------------------
>
> Message: 13
> Date: Mon, 23 Jan 2006 17:40:50 -0700
> From: IGOR NASONKIN
> Subject: Re: [Histonet] Human specific markers
> To: Jim Manavis
> Cc: Histonet
> Message-ID: <3a4109cfaf1.43d51522@jhmimail.jhmi.edu>
> Content-Type: text/plain; charset=us-ascii
>
>
> Jim,
> we use HNu (Human nuclei AB) from Chemicon; very robust, stains
> well and very specific.
> Staining is nuclear.
>
> Dr. Igor O. Nasonkin Ph.D.
> Postdoctoral Fellow
> Pathology/Neuropathology
> Johns Hopkins University
> Ross Research Bldg Rm 563
> 720 Rutland Ave
> Baltimore MD 21205
> tel: 617-388-4104
> Igor.Nasonkin@jhmi.edu
>
> ----- Original Message -----
> From: Jim Manavis
> Date: Monday, January 23, 2006 2:53 pm
> Subject: [Histonet] Human specific markers
>
>> Can anyone suggest a human specific marker for use in
>> Immnohistochemistry to
>> distinguish between human and mouse cells
>>
>> Jim
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Mon, 23 Jan 2006 17:34:45 -0800 (PST)
> From: Glenn Krasinski
> Subject: [Histonet] Source for "Citrusolve"...
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <20060124013445.73779.qmail@web37107.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I am looking for a source for "citrusolve" for use instead of
> xylene in the deparaffinization of slides. Does it go by any other
> name?
>
> Many thanks.
>
> Glenn M. Krasinski
> San Diego, CA
>
>
>
>
> ---------------------------------
> Yahoo! Photos – Showcase holiday pictures in hardcover
> Photo Books. You design it and we’ll bind it!
>
> ------------------------------
>
> Message: 15
> Date: Mon, 23 Jan 2006 20:52:02 -0500
> From: "Bartlett, Jeanine"
> Subject: RE: [Histonet] Source for "Citrusolve"...
> To: "Glenn Krasinski" ,
>
> Message-ID:
>
> Content-Type: text/plain; charset="UTF-8"
>
> It's Citrisolve and Amity International carries it....probably some
> others do as well.
>
> http://ascenaa2.miniserver.com/~amity/products/citrisolve.htm
>
> Jeanine Bartlett
> CDC, Atlanta
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Glenn
> Krasinski
> Sent: Mon 1/23/2006 8:34 PM
> To: histonet@lists.utsouthwestern.edu
> Cc:
> Subject: [Histonet] Source for "Citrusolve"...
>
>
>
> I am looking for a source for "citrusolve" for use instead of
> xylene in the deparaffinization of slides. Does it go by any other
> name?
>
> Many thanks.
>
> Glenn M. Krasinski
> San Diego, CA
>
>
>
>
> ---------------------------------
> Yahoo! Photos – Showcase holiday pictures in hardcover
> Photo Books. You design it and we’ll bind it!
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 16
> Date: Tue, 24 Jan 2006 10:07:50 +0100 (CET)
> From: eva-moeller@arcor.de
> Subject: [Histonet] Aw: BrdU Staining HCl pretreat
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> <4566989.1138093670423.JavaMail.ngmail@webmail-07.arcor-online.net>
> Content-Type: text/plain; charset=ISO-8859-1
>
>
>
> hello again,
> I forgot to mention that I cook the slices in citrate buffer for 5
> min (800 Watts in microwave) before applying the antibody.
> I am now trying to do the HCl method instead.
> I will report my results.
> Thanks for helping me.
> Eva
>
> Eva Moeller
> Ruhr-Universitaet Bochum
> Germany
>
> Machen Sie aus 14 Cent spielend bis zu 100 Euro!
> Die neue Gaming-Area von Arcor - über 50 Onlinespiele im Angebot.
> http://www.arcor.de/rd/emf-gaming-1
>
>
>
> ------------------------------
>
> Message: 17
> Date: Tue, 24 Jan 2006 07:53:19 -0600
> From: "GUTIERREZ, JUAN"
> Subject: RE: [Histonet] H.pylori immunos on gastric biopsies
> To: "Cazares, Ruth" ,
>
> Message-ID:
>
> Content-Type: text/plain; charset="US-ASCII"
>
> We do immunos on all gastric biopsies. We had some cases that looked
> negative by giemsa stain, yet the clinical hx suggested otherwise.
> When
> we ran the immunos on these so called negatives, we were shocked to
> see
> the amount of organisms we had missed. Do you want to take that
> chance?
> I always ask myself: what if this was my biopsy? Something to think
> about.
>
> Juan C. Gutierrez, HT(ASCP)
> Histology Laboratory Supervisor
> (210)704-2533
>
> My opinions are my own and do not reflect those of my employer. Long
> live free speech!
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> Cazares,
> Ruth
> Sent: Monday, January 23, 2006 3:34 PM
> To: histonet@pathology.swmed.edu
> Subject: [Histonet] H.pylori immunos on gastric biopsies
>
> Hello Histonetters,
>
>
>
> I was wondering if anyone out there is doing immuno staining routinely
> on all gastric bx's? We are considering this in my lab, but I have my
> reservations. If some of you do, how many a week? And those who
> don't,
> why not? I will be doing a literature search on this topic, but if
> anyone knows of a specific article I sure would appreciate it if you
> could share it with me.
>
>
>
> Thank you,
>
>
>
> Ruth Cazares
>
>
>
>
>
> *** Confidentiality Statement ***
> This e-mail is intended only for the use of the individual or
> entity to
> which it is addressed and may contain information that is
> privileged and
> confidential. If the reader of this message is not the intended
> recipient, please notify the sender immediately by replying to this
> message and then delete it from your system. Any review,
> dissemination,
> distribution, or reproduction of this message by unintended recipients
> is strictly prohibited and may be subject to legal restriction.
>
>
> Thank you for your cooperation.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 18
> Date: Tue, 24 Jan 2006 09:24:33 -0500
> From: bsylinda@aol.com
> Subject: [Histonet] CAP Question about Microwave
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <8C7EF090810EA74-968-20369@mblk-d37.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Histoland,
> I am writing to ask if all labs are using lab approved microwaves
> and have them vented to an outside source. Are there still labs
> using microwaves from local retailers. If not how are you
> addressing the new CAp guidelines on checklist.
>
> Thanks in advance,
> Sylinda Battle, HT (ASCP)
>
>
> ------------------------------
>
> Message: 19
> Date: Tue, 24 Jan 2006 09:39:44 -0500
> From: Tim Wheelock
> Subject: [Histonet] UN-SUBSCRIBE PLEASE
> To: "'histonet@lists.utsouthwestern.edu'"
>
> Message-ID: <43D63C30.1060105@mclean.harvard.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> I will be going on vacation.
> Please unsubscribe me.
>
> Thanks
>
>
> Any information, including protected health information (PHI),
> transmitted
> in this email is intended only for the person or entity to which
> it is
> addressed and may contain information that is privileged,
> confidential and or
> exempt from disclosure under applicable Federal or State law. Any
> review,
> retransmission, dissemination or other use of or taking of any
> action in
> reliance upon, protected health information (PHI) by persons or
> entities other
> than the intended recipient is prohibited. If you received this
> email in error,
> please contact the sender and delete the material from any computer.
>
>
>
> ------------------------------
>
> Message: 20
> Date: Tue, 24 Jan 2006 10:05:28 -0500
> From: "Slyter, Rodney"
> Subject: [Histonet] job opening
> To:
> Cc: "Hedgepeth, Alice" , "Goodrich, Jesse"
>
> Message-ID:
>
> <95B1FC82E0F69D4FB1D2C04AAAF6CD721C7B1C@rivhsexchange.rhs.rivhs.local>
> Content-Type: text/plain; charset="iso-8859-1"
>
> There is an immediate opening for a histotech at riverside regional
> medical center in Newport news Virginia. This is a full-time
> morning position for a certified or eligible histotech. We provide
> a wide variety of tests including a wide battery of immunos, iSH
> and FISH. We do approx. 16,000 specimens a year both large
> resections and biopsies. There is no weekend or call coverage.
> There are two other full time techs, two full time lab aids, and a
> certified PA who is also a certified histotech. You can email me
> directly or visit the Riverside regional medical center website for
> more information.
>
> Rod Slyter, HTL(ASCP), PA(AAPA)
> Anatomical Pathology Manager
> Riverside Regional Medical Center
>
>
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any
> attachments, is for the sole use of the intended recipient(s) and
> may contain confidential and privileged information. Any
> unauthorized review, use, disclosure or distribution is prohibited.
> If you are not the intended recipient, please contact the sender by
> reply e-mail and destroy all copies of the original message.
>
> ------------------------------
>
> Message: 21
> Date: Tue, 24 Jan 2006 10:06:52 -0500
> From: kjsavage@buffalo.edu
> Subject: [Histonet] (no subject)
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <1138115212.43d6428c1aea3@mail3.buffalo.edu>
> Content-Type: text/plain
>
> Greetings all,
>
> I am having a difficult time trying to make methacarn fixative. I
> have
> searched the list archives and found a posting in May 2005 from
> someone
> that was having the same problem I am facing: a white precipitate
> forming. In one of the responses to his posting it was suggested to
> use glass only (pipets, vials etc...). I have done that, and still
> the
> precipitate forms. I have also used all new reagents (chloroform,
> methanol, acetic acid) and still the precipitate forms. Does anyone
> have some more suggestions as to what is going wrong? Thanks, Kathy
>
> Kathy Savage, PhD
> Dept. of Exercise and Nutrition Sciences
> SUNY-University at Buffalo
>
>
>
> ------------------------------
>
> Message: 22
> Date: Tue, 24 Jan 2006 09:29:19 -0600
> From: Bill Blank
> Subject: RE: [Histonet] H.pylori immunos on gastric biopsies
> To:
> Message-ID:
> Content-Type: text/plain; charset="us-ascii" ; format="flowed"
>
> IMO, we take this detecting H. pylori thing too seriously. A rational
> physician treats the patient and not any lab test. If I had symptoms
> of H. pylori, if my stomach looked inflamed, I would want to be
> treated for H. pylori irregardless of their presence on a biopsy.
> (Well, maybe as there is some evidence that treating antral H. pylori
> may increase the risk of GE junction adenocarcinoma)
>
> I consider immunos on gastric biopsies to be overkill and a waste of
> health care dollars.
>
> BIll
>
> At 7:53 AM -0600 1/24/06, GUTIERREZ, JUAN wrote:
>> We do immunos on all gastric biopsies. We had some cases that looked
>> negative by giemsa stain, yet the clinical hx suggested
>> otherwise. When
>> we ran the immunos on these so called negatives, we were shocked
>> to see
>> the amount of organisms we had missed. Do you want to take that
>> chance?
>> I always ask myself: what if this was my biopsy? Something to think
>> about.
>
> --
> _____________________________
> Bill Blank
> http://kernunnos.com (Celtic studies and numismatics)
> OBOD's Message board: http://www.druidry.org/board
>
>
>
>
>
>
> ------------------------------
>
> Message: 23
> Date: Tue, 24 Jan 2006 08:50:35 -0700
> From: Gayle Callis
> Subject: RE: [Histonet] How to get good cross sections of the mouse
> small intestine
> To: "Monfils, Paul" ,
> Histonet@lists.utsouthwestern.edu
> Message-ID:
> <6.0.0.22.1.20060124083902.01b439c8@gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Paul,
>
> Excellent suggestion - for pinning down the samples, I suggest using
> disposable hypodermic needles - the latter do NOT rust or corrode in
> aqueous based NBF - needles are stainless steel. Tiny needles (24
> or less
> gauge) for this purpose and not tear the ends of the intestine to
> bits
> with larger gauge.
>
> The Gordian knot description was so true and our loops of intestine
> always
> were filled with mouse feces!!! Once fixed, couldn't removed -
> microtomy
> was no fun and sections looked terrible.
>
> You can also use cork strips (purchased from hardware store, very
> cheap)
> then toss them out later. We would stand these on end in a deep
> bucket
> with lid - tagged carefully. And a cheap pan with a lid is the
> cake pan
> sizes or larger, with lids - Rubbermaid or some other plastic.
> Very nice
> to reach into a pan and get the samples - loved this idea.
>
>
>
> At 04:44 PM 1/23/2006, you wrote:
>> In addition to Gayle's suggestions, you might want to
>> physically
>> hold the intestine straight while the formalin fixes and hardens
>> it. After
>> injecting formalin as Gayle said, I pin one end of it in a wax-lined
>> dissection pan, then stretch it gently, just enough to straighten
>> it, and
>> pin the other end to keep it straight, then cover it with
>> formalin. People
>> sometimes send me intestine specimens which they have simply
>> removed from
>> the animal and dropped into formalin, resulting in a hardened
>> gordian knot,
>> all parts of which are curved to some degree, so that pieces cut
>> from the
>> intestine are shaped like parentheses. It's difficult to get a good
>> perpendicular section of a curved object. If you don't have a
>> dissecting
>> pan you can easily make one; or you can just pin the specimen onto
>> a strip
>> of heavy corrugated cardbord or soft wood and drop it specimen
>> side down
>> into a dish of formalin.
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Gayle Callis HTL, HT, MT(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University
> Bozeman MT 59717
>
>
>
>
> ------------------------------
>
> Message: 24
> Date: Tue, 24 Jan 2006 10:57:06 -0500
> From: bsylinda@aol.com
> Subject: Re: [Histonet] CAP Question about Microwave
> To: bsylinda@aol.com, histonet@lists.utsouthwestern.edu
> Message-ID: <8C7EF15F587F78E-A58-150A6@FWM-D06.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
> An additionan to comment. This microwave is only used for special
> stains no tissuse processing.
> Thanks again,
> sylinda
>
> -----Original Message-----
> From: bsylinda@aol.com
> To: histonet@lists.utsouthwestern.edu
> Sent: Tue, 24 Jan 2006 09:24:33 -0500
> Subject: [Histonet] CAP Question about Microwave
>
>
> Hello Histoland,
> I am writing to ask if all labs are using lab approved microwaves
> and have them
> vented to an outside source. Are there still labs using microwaves
> from local
> retailers. If not how are you addressing the new CAp guidelines on
> checklist.
>
> Thanks in advance,
> Sylinda Battle, HT (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 25
> Date: Tue, 24 Jan 2006 10:17:45 -0600
> From: "Smith, Jeffery D. \(HSC\)"
> Subject: RE: [Histonet] H.pylori immunos on gastric biopsies
> To: "Bill Blank" ,
>
> Message-ID:
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I have to somewhat agree. Immunos for H. pylori seems to be
> overkill. We routinely stain for H. pylori using a Giemsa on all
> GI biopsies.
>
> ________________________________
>
> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bill
> Blank
> Sent: Tue 1/24/2006 9:29 AM
> To: histonet@pathology.swmed.edu
> Subject: RE: [Histonet] H.pylori immunos on gastric biopsies
>
>
>
> IMO, we take this detecting H. pylori thing too seriously. A rational
> physician treats the patient and not any lab test. If I had symptoms
> of H. pylori, if my stomach looked inflamed, I would want to be
> treated for H. pylori irregardless of their presence on a biopsy.
> (Well, maybe as there is some evidence that treating antral H. pylori
> may increase the risk of GE junction adenocarcinoma)
>
> I consider immunos on gastric biopsies to be overkill and a waste of
> health care dollars.
>
> BIll
>
> At 7:53 AM -0600 1/24/06, GUTIERREZ, JUAN wrote:
>> We do immunos on all gastric biopsies. We had some cases that looked
>> negative by giemsa stain, yet the clinical hx suggested
>> otherwise. When
>> we ran the immunos on these so called negatives, we were shocked
>> to see
>> the amount of organisms we had missed. Do you want to take that
>> chance?
>> I always ask myself: what if this was my biopsy? Something to think
>> about.
>
> --
> _____________________________
> Bill Blank
> http://kernunnos.com (Celtic studies and numismatics)
> OBOD's Message board: http://www.druidry.org/board
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 26
> Date: Tue, 24 Jan 2006 11:27:14 -0500
> From: "Sue Barnes"
> Subject: [Histonet] Health Testing when using xylene
> To:
> Message-ID:
> Content-Type: text/plain; charset="Windows-1252"
>
>
> Does anyone in the histology world have health testing for techs
> who use xylene?
> What testing is done?
> Is there printed regulations for suggested health testing?
> Any help on this would be appreciated.
>
> Thanks
> Sue Barnes
> East Liverpool City Hospital
> East Liverpool, Ohio
>
>
> ------------------------------
>
> Message: 27
> Date: Tue, 24 Jan 2006 11:32:29 -0500
> From: Phil McArdle
> Subject: Re: [Histonet] CAP Question about Microwave
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <43D6569D.5010309@ebsciences.com>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Hello:
>
> If you want some "vendor" input, please feel free to contact me.
>
> Best regards,
>
> Phil McArdle
>
> --
> Phil McArdle
> Microwave Product Manager
> Energy Beam Sciences, Inc.
> Tel: 800.992.9037 x 341
> Fax: 860.653.0422
> PMcardle@ebsciences.com
> www.ebsciences.com
> "ADDING BRILLIANCE TO YOUR VISION"
>
>
>
>
> ------------------------------
>
> Message: 28
> Date: Tue, 24 Jan 2006 08:48:28 -0800 (PST)
> From: Rene J Buesa
> Subject: Re: [Histonet] Health Testing when using xylene
> To: Sue Barnes , histonet@lists.utsouthwestern.edu
> Message-ID: <20060124164828.11569.qmail@web61214.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Sue:
> I used to test for formaldehyde, glutaraldehyde, xylene and the
> air flow level of the fumes hoods.
> The fumes hoods had simple air gauges tha were checked daily and
> the results written in a log in the hood door.
> For the xylene (as well as the others) we used pasive monitoring
> badges worn during 8 hours (to determine Time Weighed Average or
> TWA) or during 15 minutes (to determine Short Term Exposure Level
> or STEL).
> Some of the badges were developed in our lab and others were sent
> to an outside laboratory for confirmation of results.
> After 8 years of cummulative data with values below OSHA levels,
> and because this was a very expensive program, we contracted an
> outside company that did the monitoring only in the areas and we
> stopped doing the personal monitoring.
> Hope this will help you!.
> René J.
>
> Sue Barnes wrote:
>
> Does anyone in the histology world have health testing for techs
> who use xylene?
> What testing is done?
> Is there printed regulations for suggested health testing?
> Any help on this would be appreciated.
>
> Thanks
> Sue Barnes
> East Liverpool City Hospital
> East Liverpool, Ohio
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ---------------------------------
> Yahoo! Photos
> Ring in the New Year with Photo Calendars. Add photos, events,
> holidays, whatever.
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 26, Issue 27
> ****************************************
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