Sharon,
About your IHC slides losing tissues during processing: It's probably what 
Rene and Linda said - preheating is necessary. But I have heard that the 
treated slides purchased from vendors lose their stickiness over time. You 
might check other lots or other vendors to see if you still have problems. 
Were these slides brand new? Has anybody else had problems with old 
pretreated slides losing their stickiness? 
Mel
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
Edmond, OK 73034
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