[Histonet] DNA PCR Question
Many thanks to the histonetters that answered my question about inverts in
mud but I have another question in a field that I know nothing about. Why
is it when people see paraffin they come running to the histologist? O.K.
that's not the question. Here it is. I have a researcher (a different one
from last time) that is trying to extract shellfish pathogen DNA from a
paraffin embedded sample. He is getting background fluorescence in a RTPCR
using DNA extracted from this paraffin embedded tissue. The tissue was
originally fixed in Davidsons fixative. He is using a Takara Dexpat kit and
he wants to know how to solve this problem using a Taqman MGB (FAM) probe?
Any suggestions would be great and much appreciated.
Fisheries and Oceans Canada
Pacific Biological Station
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