[Histonet] BrdU Staining HCl pretreat
Did you pretreat the sample with 2N or 4N HCl?
Melville B. Vaughan, Ph. D.
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
I am trying to do a BrdU staining on frozen tissue slices (already fixed
with PFA 4%) for weeks.
The signal is restricted to the rim of the core and not within it. I am
staining with anti-BrdU by the Roche detection kit. I already tried their
protocol but that did not work on my fixed slices.
I dry the slices then rehydrate for 1 h. After that I apply the 1st
antibody for 30 min 37°C.
Thanks for support.
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