Fw: [Histonet] Invertebrates in Sediment
----- Original Message -----
From: "Bryan Hewlett"
Sent: Thursday, January 19, 2006 1:14 PM
Subject: Re: [Histonet] Invertebrates in Sediment
> The third edition of "Staining Procedures used by the Biological Stain
> Commission" edited by George Clark,
> gives a procedure (Walton 1952) for staining foraminiferans alive at the
> time of fixation of marine sediments "without incidental staining of
> organic or inorganic debris".
> The concentration of Rose Bengal is given as 0.1% aqueous and the staining
> time as 10 minutes.
> There is a footnote that the concentration of dye is not critical.
> Since the dye is also a fluorochrome, enumeration may be easier at low
> power by fluorescence microscopy.
> ----- Original Message -----
> Sent: Thursday, January 19, 2006 12:24 PM
> Subject: [Histonet] Invertebrates in Sediment
>> Hello Histonetters,
>> Thanks in advance for spending any time with this query! I have a
>> researcher that has collected sediment samples, preserved with NBF, and
>> wants to stain any invertebrates in the sediment so that they can be
>> enumerated and identified. The researcher wants to use rose bengal but
>> doesn't know at what concentration and neither do I. Can anyone help?
>> Bill Bennett
>> Fisheries and Oceans Canada
>> Pacific Biological Station
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