Re: [Histonet] neurite visualization in cell culture
Carri & Ebendal 1989
Coomassie brilliant blue R250 (CI 42660): 0.1g
Methanol: 25 ml
Acetic acid: 10 ml
Water to 100 ml
Stain for 20-30 minutes.
For details see the original paper in Stain
Technology 64:50-52.
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-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan[AT]uwo.ca
http://publish.uwo.ca/~jkiernan/
http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Caroline Bass wrote:
>
> Hello,
>
> I am doing a neurite outgrowth assay in vitro and I need to add some
> contrast in order to visualize the neurites. Is there a protocol for
> staining entire cells that will bring out this detail? I just need
> to darken the entire cell and the neurite. I have heard of some
> people using commassie blue for this purpose. Does anyone have a
> protocol for this?
>
> Thanks,
>
> Caroline Bass
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