Re: [Histonet] mallory-Aaan Trichrome

From:Rene J Buesa

Hi Steve:
  Yes, Mallory-Azan stains α cells RED, ▀ cells YELLOW, and delta cells BLUE; many years ago I used this stain as well as Maximov's modification (1909) of Zenker's.
  Formula: water → 250 mL + mercuric chloride → 12.5 g + potassium dichromate → 6.25 g + 40% formaldehyde → 25 mL + sodium sulfate → 2.5 g
  RenÚ J. 

Steven Coakley  wrote:
  Does anyone know about this pancreas islet cell stain and whether it stains A,B and D cells? Has anyone uses Maximows modification zenkers fixitive for these cells also.?

Thanks,

Steve


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