Re: [Histonet] Reihard, please help CD4, CD8 in FFPE Bone Marrow

From:Lori Richey

We use both CD4 and CD8 on FFPE sections.  We had problems with the CD4 
until we changed our quenching step to 0.5% H2O2 in MEOH for 10 min. The 
pretreatment for both is 15 min microwave in pH 8 EDTA.

Thomas Pier wrote:

>I am looking for Reinhard von Wasielewski.  I recently saw a post
>stating that he had working protocols for CD4 and CD8 that even worked
>in decalcified bone marrow.  CD4 and CD8 in FFPE, decalcified bone
>marrow is exactly what I'm having problems with.  I have been banging my
>head against the wall with this for some time now.  I would really
>appreciate any help I could get on this.  Thank you very much.
>Tom Pier
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