Re: [Histonet] Reihard, please help CD4, CD8 in FFPE Bone Marrow
We use both CD4 and CD8 on FFPE sections. We had problems with the CD4
until we changed our quenching step to 0.5% H2O2 in MEOH for 10 min. The
pretreatment for both is 15 min microwave in pH 8 EDTA.
Thomas Pier wrote:
>I am looking for Reinhard von Wasielewski. I recently saw a post
>stating that he had working protocols for CD4 and CD8 that even worked
>in decalcified bone marrow. CD4 and CD8 in FFPE, decalcified bone
>marrow is exactly what I'm having problems with. I have been banging my
>head against the wall with this for some time now. I would really
>appreciate any help I could get on this. Thank you very much.
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