Re: [Histonet] Ki-67 or PCNA on FFPE primate tissue

From:antje.marcantonio@novartis.com

Roger,

we do successfully IHC for Ki67 on primate FFPE tissue  with the mouse 
monoclonal anti-Ki67, clone MIB5 from DakoCytomation.
Antigen retrieval 98 degrees C with citrate buffer, pH 6.0 for 20 min 
Primary ab dilution 1/100 for 1 hour , or 1/500 for over night incubation.
Hope this helps, 


Antje Marcantonio
Novartis Pharma AG
PH214217, AT TX: LAB. WIECZOREK
CHBS, WSJ-386.5.55
Novartis Pharma AG
Lichtstrasse 35
CH-4056 Basel
Switzerland
Phone: +41 61 3246730
Fax: +41 61 3247534
Email : antje.marcantonio@novartis.com
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>