Re: [Histonet] H&E staining in solid tumors...

From:"Andrea T. Hooper"

This is potentially true of course but pink could also be stromal 
areas (sometimes nuclei so small they are hard to detect) or more 
relevantly areas of intense collagen/matrix deposition. Something to 
consider when setting something like this up.

>Can H&E staining be used to determine necrosis in solid tumors 
>treated with agents?  If yes, then would pink only staining signify 
>areas of necrosis and purple/pink staining areas of live/intact 
>cells? Could precent necrosis be then calculated using the areas of 
>pink only versus purple/pink?
>   Glenn M. Krasinski
>   San Diego, CA
>Yahoo! Photos
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