[Histonet] sucrose liver

From:Steven Coakley

  Good morning everyone,   If anyone out there cryo-sections sucrose infiltrated fixed liver I'd like to know what temp there are sectioning at best.  The mouse liver is fixed at 4C in formalin/PBS for 6 hours the 25-30% sucrose overnight also at 4C.  I thought sucrose infiltrated tissue should section closer to -25C.  Our chatter at anything above -18C.

 Yahoo! DSL Something to write home about. Just $16.99/mo. or less
Histonet mailing list

<< Previous Message | Next Message >>