Good morning everyone,   If anyone out there cryo-sections sucrose infiltrated fixed liver I'd like to know what temp there are sectioning at best.  The mouse liver is fixed at 4C in formalin/PBS for 6 hours the 25-30% sucrose overnight also at 4C.  I thought sucrose infiltrated tissue should section closer to -25C.  Our chatter at anything above -18C.
   
  Steve

		
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