[Histonet] RE Processing/Embedding
We often take cassettes off the processor and leave them to solidify while
other duties are carried out. We do this out of necessity when busy and then
melt the wax and embed the tissues at a convenient time. We have never had
any problems with morphology or shrinkage.
Alasdair Wilson
----- Original Message -----
From:
To:
Sent: Friday, January 06, 2006 5:58 PM
Subject: Histonet Digest, Vol 26, Issue 4
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> Today's Topics:
>
> 1. Re: document for recording special stain results for quality
> control (Rene J Buesa)
> 2. Smear preps (Loralei Dewe)
> 3. Re: document for recording special stain results for quality
> control (Linda Blazek)
> 4. Re:Plastic sectioning (Gayle Callis)
> 5. RE: Happy New Year's and competancy/volume questions
> (Kinsley, David)
> 6. gross room (Tom Wells)
> 7. 50 micron mouse brain (Atoska S. Gentry)
> 8. Job Opportunity (lena spencer)
> 9. Re: Number of GI biopsy sections on a slide for
> pediatrichospitals. (Joe Nocito)
> 10. Re: CD4 and CD8 in mouse / human FFPE (Kuo Christy)
> 11. RE: (Louis Apuzzio)
> 12. RE: optimal thickness for cutting of IHC sections
> (C.M. van der Loos)
> 13. Looking for a home... (Ford Royer)
> 14. slide dryer (dfleisch@bidneedham.org)
> 15. Processing/embedding question - your opinion (foley1)
> 16. RE: Processing/embedding question - your opinion (Allen, Rhonda)
> 17. RE: Price quotes on refurbished lab equipment/ Start Up Lab
> (mprice26@juno.com)
> 18. Re: Processing/embedding question - your opinion (Rene J Buesa)
> 19. Microwave slide drying vs. conventional slide drying
> (lharris@samhealth.org)
> 20. sucrose liver (Steven Coakley)
> 21. HTL certification (Hermina Borgerink)
> 22. RE: Processing/embedding question - your opinion (Bonnie Whitaker)
> 23. RE: Microwave slide drying vs. conventional slide drying
> (Laurie Colbert)
> 24. RE: Processing/embedding question - your opinion (Rene J Buesa)
> 25. RE: Processing/embedding question - your opinion (Allen, Rhonda)
> 26. microwave tissue processing (Harrison, Sandra C.)
> 27. RE: Processing/embedding question - your opinion
> (Trajkovic, Dusko)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 5 Jan 2006 10:00:23 -0800 (PST)
> From: Rene J Buesa
> Subject: Re: [Histonet] document for recording special stain results
> for quality control
> To: "Adams, Nancy" ,
> histonet@lists.utsouthwestern.edu
> Message-ID: <20060105180023.17687.qmail@web61221.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi Nancy:
> We used to "batch" special stains (1 positive control for slides with the
> same procedure, either IHC, HC or even Her-2new), so we had a "common"
> form where we used to write the date, the test we batched, and the cases
> numbers included in the batch.
> We checked if the control reacted as expected, attached the form and the
> control to the batched slides and gave them to the pathologist.
> The form was originally signed by the histotech who did the test and it
> was also signed by the pathologist.
> All the forms were filed by the type of test (Steiner, IHCs on the day,
> PAS, etc.) and were available for inspection.
> In our last CAP inspection we were told that this was a good procedure.
> I hope this will help you!
> René J.
>
> "Adams, Nancy" wrote:
> Does anyone have a good document for recording this information that
> they'd be willing to share? I'm in the process of getting ready for a
> CAP inspection.
>
> Thanks
>
> Nancy Rutledge
>
> Falmouth Hospital
>
> Falmouth,
>
> MA
>
>
>
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> ------------------------------
>
> Message: 2
> Date: Thu, 5 Jan 2006 10:11:58 -0800 (PST)
> From: Loralei Dewe
> Subject: [Histonet] Smear preps
> To: "Van Eyck, Deb"
> Cc: "' histonet@lists.utsouthwestern.edu'"
>
> Message-ID:
> Content-Type: TEXT/PLAIN; charset=US-ASCII
>
>
> I need to do some smears from frozen tissue. I've never done them before
> but they don't sound all that tough. Anyone got a good protocol to share
> with me?
>
> Cheers,
> Loralei Dewe
> UC Davis Vet Hospital
>
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 05 Jan 2006 13:12:52 -0500
> From: "Linda Blazek"
> Subject: Re: [Histonet] document for recording special stain results
> for quality control
> To: nadams@CapeCodHealth.org, histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset=US-ASCII
>
> I forgot to add something to my previous note. We run batch controls also
> as Rene does but we have a control label ie: GMS #1, IRON #1 etc. The
> control number is also dictated on the pathology report. That way we can
> always go back to the correct control slide - Linda
>
>
> Linda Blazek, HT (ASCP)
> Department of Pathology
> Children's Medical Center
> Dayton, Ohio 45404
> (937) 641-3358
> fax (937)641-5482
> blazekl@childrensdayton.org
>
>
>>>> "Adams, Nancy" 01/05/2006 11:13 AM >>>
>
> Does anyone have a good document for recording this information that
> they'd be willing to share? I'm in the process of getting ready for a
> CAP inspection.
>
> Thanks
>
> Nancy Rutledge
>
> Falmouth Hospital
>
> Falmouth,
>
> MA
>
>
>
> ************************************************************
> This email and any files transmitted with it are confidential. And
> intended solely for the use of the individual or entity to whom they are
> addressed. If you have received this email in error please contact the
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>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 05 Jan 2006 13:22:24 -0700
> From: Gayle Callis
> Subject: [Histonet] Re:Plastic sectioning
> To: , Histonet@lists.utsouthwestern.edu
> Message-ID:
> <6.0.0.22.1.20060105130946.01b60008@gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Sunny,
>
> You have a tough problem here. 30 um thick GMA sections may be too thick
> for this embedding media. GMA plastic was initially designed for doing
> THIN sections at 1 to 3 micrometers. It tends to be brittle when cutting
> thick sections and one might have to use a very sharp tungsten carbide
> tipped knife in order to get any thick section off a GMA block. One could
> try to adjust the plasticizer by increasing its concentration to make the
> GMA more pliable. The knife, no matter how sharp, may not actually slice
> through the plastic, but force its way through in such a way that the
> plastic shatters i.e. a shearing effect and not really have a clean cut.
>
> Patsy Ruegg published an inhouse method for GMA, rather than buy kits -
> that way she could adjust the ingredients accordingly for softer or harder
> GMA.
>
> If the tissues are thicker than 1 to 2 mm when processing and embedding,
> infiltration may be a problem unless extended time is used and done at 4C
> (
> refrigerator) and polymerization might have to controlled with cold
> temperatures in order to slow it down for continuous or steady progression
> of polymerizing. That can be done at 4C also.
>
> At 12:58 AM 1/5/2006, you wrote:
>>Dear Sunny,
>>
>>First about the knife marks. To me it seems that you are using a blunt
>>knife. Try a new/sharp one.
>>About the shattering: I don't know how youre blocks look like. Is the GMA
>>well impregnated into the brain-tissue, for else this could explain the
>>problem. If this is not the case (I don't think, else you would have
>>mentioned it) then perhaps you can try to use some sort of tape. Stick it
>>onto the block and then cut you're slice. I've tried it and it worked in
>>my case. The only problem then is how to remove the tape without damaging
>>the slice (unless you stain the slices when still sticked to the tape).
>>There are also commercial tapes specially for this technique (see for
>>instance this link:
>>http://www.alphelys.com/site/us/pHGP_CoupesParaffine.htm).
>
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 5 Jan 2006 16:32:50 -0500
> From: "Kinsley, David"
> Subject: RE: [Histonet] Happy New Year's and competancy/volume
> questions
> To: 'Rene J Buesa' , "Van Eyck, Deb"
> , "' histonet@lists.utsouthwestern.edu'"
>
> Message-ID:
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi all,
>
> It seems to me that the competencies below are for human tissues in a
> hospital/clinical setting. I was wondering if there are
> competency/productivity standards for research histology on animal tissues
> particularly mouse and rat species, paraffin and frozen. We are looking
> to
> establish some base guidelines for our lab. Any suggestions are
> appreciated.
>
> thanks
>
> Dave
>
>
> -----Original Message-----
> From: Rene J Buesa [mailto:rjbuesa@yahoo.com]
> Sent: Wednesday, January 04, 2006 6:33 PM
> To: Van Eyck, Deb; ' histonet@lists.utsouthwestern.edu'
> Subject: Re: [Histonet] Happy New Year's and competancy/volume questions
>
>
> Hi Deb:
> In 3 labs I wrote competencies for the expectation for sectoning was set
> at 25 blocks/hour and for embedding at 60 blocks/hour.
> Each histotech was evaluated on that basis and their individual averages
> were
> rated (as percentages) against the expectation. Those with averages above
> expectations were evaluated better (and received salary compensations
> accordingly). Nobody was considered "proficient" until their individual
> average reached the expectation consistently.
> For time utilization (ratio between time with recorded tasks versus
> logged
> time) the expectation was 75 % (i.e. 6 h with logged tasks agains 8 h/day
> per shift).
> In the Dec./04 issue of the Journal of Histotechnology there are averages
> for every imaginable task within the histology lab as a result of a survey
> of 12 labs. with annual volumes ranging from 4800 to 77000 cases/year. If
> your lab is within that range this information could be used by you.
> I hope this will help you!
> René J.
>
> "Van Eyck, Deb" wrote:
>
> Hi all,
>
> If anyone has information they would like to share I would appreciate it.
> We
> are a hospital lab and we are updating doing competencies. We are
> interested
> in Volumes and expectations for embedding, cutting etc, that take into
> account differeing experience levels, training and quality
> expectations---does anyone have a good system or have good references or
> articles that discuss this issue? thanks for your input. Deb
>
>
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> ------------------------------
>
> Message: 6
> Date: Thu, 5 Jan 2006 14:06:00 -0800
> From: Tom Wells
> Subject: [Histonet] gross room
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>
> Content-Type: text/plain; charset="US-ASCII"
>
> I am going to be discussing the gross room and prosecting with my medical
> laboratory technology students soon. This is an area that seems to be
> rather poorly documented. Do any of you have gross room manuals that you
> wouldn't mind sharing with me? Are there any web sites which discuss
> grossing for new residents? Are there any pathology sites that have
> established standards for grossing etc. Any help would be gratefully
> appreciated. Thanks. Tom
>
> ------------------------------
>
> Message: 7
> Date: Thu, 05 Jan 2006 16:36:05 -0600
> From: "Atoska S. Gentry"
> Subject: [Histonet] 50 micron mouse brain
> To: Histonet
> Message-ID:
> <6.0.1.1.0.20060105163140.01acf798@mailhost.vetmed.auburn.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Hello, will someone please give me pointers on obtaining good 50u sections
> of PFA drop fixed and/or immersion fixed coronal paraffin sections of
> mouse
> brain? As I section they tend to roll up in tight rolls. I've tried
> sectioning at room temp. vs. chilled sections. Any assistance provided
> will
> be greatly appreciated. Thanks, Atoska
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Thu, 5 Jan 2006 18:55:31 -0500
> From: "lena spencer"
> Subject: [Histonet] Job Opportunity
> To:
> Message-ID: <005401c61253$8311b880$3379df0c@org.insightbb.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Electron Microscopy Job Opportunity
> Full Time/Day Shift
> some histology duties required
>
> Norton Healthcare
> Louisville, Kentucky
> Contact: Mary Beth Knight, MT(ASCP)HTL
> marybeth.knight@nortonhealthcare.org
> 502-629-7884
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Thu, 5 Jan 2006 21:35:07 -0600
> From: "Joe Nocito"
> Subject: Re: [Histonet] Number of GI biopsy sections on a slide for
> pediatrichospitals.
> To: "Foshey, Annette" ,
>
> Message-ID: <007901c61272$30e4f1c0$e8bd0b43@yourxhtr8hvc4p>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=original
>
> Annette,
> we cut 3 levels on one slide with 2 sections per level
>
> Joe Nocito BS, HT(ASCP)QIHC
> Histology Manager
> Pathology Reference Lab
> San Antonio, TX
> ----- Original Message -----
> From: "Foshey, Annette"
> To:
> Sent: Wednesday, January 04, 2006 3:50 PM
> Subject: [Histonet] Number of GI biopsy sections on a slide for
> pediatrichospitals.
>
>
>
> I am inquiring what the "standard" is for the number of GI biopsy
> sections that are routinely put on slides for review. We currently cut
> two slides with two levels on each slide. The total number of sections
> is between 6-8 sections on each slide.
>
> Thanks,
> Annette Foshey, HT
> Team Leader in Histology
> Children's Hospital of Wisconsin
>
>
> **************************
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> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
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>
>
> ------------------------------
>
> Message: 10
> Date: Fri, 6 Jan 2006 14:35:02 +0800
> From: Kuo Christy
> Subject: [Histonet] Re: CD4 and CD8 in mouse / human FFPE
> To: Laurie Reilly
> Cc: histonet ,
> wasielewski.reinhard.von@mh-hannover.de
> Message-ID:
> <5e4834130601052235w191fb3f9g206edcd8ac567a9e@mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi Reinhard,
> Could I also ask for a copy of your protocol, cuz the results from
> anti-CD4(Novocvastra) seems to be very unspecific on human tissue
> FFPE sections and I don't know the reason.
>
> Thank you for your help!
>
> Best wishes,
> Christy
> christy0424@gmail.com
>
> On 12/19/05, Laurie Reilly wrote:
>> Reinhard,
>> Could I please have a copy of your protocol, we have had lots of trouble
>> trying to stain with
>> CD4 and CD8 in bovine tissues.
>> Any advice you can give would be most welcome, particularly about antigen
>> retrieval.
>>
>> Hope everyone has a happy Christmas,
>> Regards,
>> Laurie.
>>
>> At 08:49 PM 18/12/2005 +0100, you wrote:
>> >Hi Patsy,
>> >we do a lot of CD4 and CD8 in human FFPE tissues, even in BM after
>> >decalification.
>> >It works very good and is daily routine in our lab. If you need advice,
>> >give me a mail.
>> >We now got some hints how both markers could work in mouse tissue, too.
>> >If
>> we
>> >succeed, we'll let you know.
>> >
>> >best regards
>> >Reinhard.
>> >
>> >
>> >
>> >PD Dr. med. Reinhard von Wasielewski
>> >
>> >
>> >_______________________________________________
>> >Histonet mailing list
>> >Histonet@lists.utsouthwestern.edu
>> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> Mr.Laurie Reilly Ph 07 4781
>> 4468
>> School of Veterinary & Biomedical Science Fax 07 4779 1526
>> James Cook University
>> Townsville Qld.
>> 4811 laurie.reilly@jcu.edu.au
>>
>> Australia.
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Fri, 6 Jan 2006 05:54:04 -0500
> From: "Louis Apuzzio"
> Subject: [Histonet] RE:
> To:
> Message-ID:
> Content-Type: text/plain; charset="iso-8859-1"
>
> Anyone in search of a Histotech, ( HT eligible) and also a Cytotech, (
> CT(ASCP),
>
> for small private laboratory and or hospital, please contact:
>
> ljapuzzio@msn.com
>
> Thank you !
>
>
> ------------------------------
>
> Message: 12
> Date: Fri, 06 Jan 2006 12:26:41 +0100
> From: "C.M. van der Loos"
> Subject: [Histonet] RE: optimal thickness for cutting of IHC sections
> To: histonet@lists.utsouthwestern.edu
> Cc: PMonfils@Lifespan.org
> Message-ID: <1d9a431dc72b.1dc72b1d9a43@amc.uva.nl>
> Content-Type: text/plain; charset="windows-1252"
>
>
> Sorry guys my message yesterday was somewhat distorted. Here it is
> again...
>
>
> Dear Paul,
>
>
> I totally agree what you wrote about IHC: it's just a surface event.
>
> Long time ago we had to prove that our (prehistoric) attempt of
> quantify due to microtome different thickness and gue on the
> staining intensity. The only thicker sections showed a higher
> non-specific b than thinner sections. See:
>
>
> CM van der Loos, MMH Marijianowski and AE Becker: Quantification in
> immu collagen types I
>
> Chris van der Loos, PhD
> Dept. of Pathology
> Academic Medical Center Meibergdreef 9
> NL-1105 AZ Amsterdam
> The Netherlands
>
>
>
>
>
> Date: Tue, 3 Jan 2006 13:09:20 -0500
> From: "Monfils, Paul" thickness for cutti sections
> To: [1]'histonet@lists.utsouthwestern.edu' I believe section
> thickness is less critical for IHC because antibo dies are
> very large molecules that don't penetrate tissue very well, so
> regardless of
> the thickness of the section, you are really only staining
> exposed
> surface of the tissue, perhaps to a depth of 2 microns or s have
> verified this by electron microscopy. This is qui standard
> histochemical procedures where a thicker sect intense
> stain because the small dye molecules pene and stain
> it all the way through.
>
>
>
>
>
>
>
>
> // Script broken into two blocks. There is a in the
> document.write bugs 4553030/31, 5047193. document.form
> var form1 = document.form1
> var form2 =
> document.form2
> var errno = 0
> arg=window.location.search;//AK
> if (!refreshing) {
> if
> (arg.length > 6 && arg.substring(0, 7) == 'bgcolor')
> color(arg.substring(8, arg.length))
> f = eval('parent.' +
> self.name + 'Init')
> if (f)
> f()
> }
> // Note: the tag is
> defined in main.js and written out when a given is created.
>
>
> /**Enable backspace for text editing*/
> if
> (NN>=5){
> setBkspEvtHndlr("input");//specifically for text
> elements. checkboxes/r setBkspEvtHndlr("textarea");
> setBkspEvtHndlr("browse");
> }
>
>
>
> References
>
> 1. 3D"javascript:main.compose('new','t='hi
>
> ------------------------------
>
> Message: 13
> Date: Fri, 6 Jan 2006 08:26:31 -0600
> From: "Ford Royer"
> Subject: [Histonet] Looking for a home...
> To: "Histonet"
> Message-ID: <002001c612cd$30f0a230$3900a8c0@fords>
> Content-Type: text/plain; charset="us-ascii"
>
> A friend has asked me to try and find a home for her retired TissueTek VIP
> (K-series) Tissue Processors.
> These would make a great back-up unit or a basic unit in a start-up lab.
>
> Contact me Off-List for details.
>
> ~ Ford
>
> Ford M. Royer, MT(ASCP)
> Minnesota Medical Specialists, Inc.
> Golden Valley, MN 55427-3601
> 763-542-8725 phone
> 888-790-9686 toll free
> 763-546-4830 fax
> email
>
>
>
> ------------------------------
>
> Message: 14
> Date: Fri, 6 Jan 2006 09:46:22 -0500
> From:
> Subject: [Histonet] slide dryer
> To:
> Message-ID:
>
> Content-Type: text/plain; charset="us-ascii"
>
> I am looking for a small slide dryer.
>
> Would greatly appreciate any suggestions.
>
>
>
> Deborah S. Fleischhacker, M.D.
>
> Pathologist
>
> Beth Israel Deaconess Hospital Needham
>
> 148 Chestnut Street
>
> Needham, MA 02492
>
> Phone: (781) 453-3087
>
> Fax: (781) 453-3097
>
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Thu, 29 Dec 2005 12:40:59 -0500
> From: foley1
> Subject: [Histonet] Processing/embedding question - your opinion
> To:
> Message-ID:
> Content-Type: text/plain; charset="US-ASCII"
>
> Does anyone routinely allow for the hot wax to drain off multiple
> cassettes
> of processed tissue and be held at room temperature for multiple days (6
> days) before embedding? What would the consequences be to morphology,
> possible immunohistochemistry and molecular (DNA) studies?
>
>
>
>
>
> ------------------------------
>
> Message: 16
> Date: Fri, 6 Jan 2006 10:19:39 -0600
> From: "Allen, Rhonda"
> Subject: RE: [Histonet] Processing/embedding question - your opinion
> To: "foley1" ,
>
> Message-ID:
>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Have done it on many occasions and never had it be a problem.
>
> Rhonda Allen
> Stowers Institute
> 1000 E. 50th street
> Kansas City, Missouri 64110
> rra@stowers-institute.org
>
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of foley1
> Sent: Thursday, December 29, 2005 11:41 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Processing/embedding question - your opinion
>
>
> Does anyone routinely allow for the hot wax to drain off multiple
> cassettes of processed tissue and be held at room temperature for
> multiple days (6
> days) before embedding? What would the consequences be to morphology,
> possible immunohistochemistry and molecular (DNA) studies?
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 17
> Date: Fri, 6 Jan 2006 16:49:27 GMT
> From: "mprice26@juno.com"
> Subject: [Histonet] RE: Price quotes on refurbished lab equipment/
> Start Up Lab
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <20060106.085006.9834.259217@webmail48.nyc.untd.com>
> Content-Type: text/plain
>
> Histonetters,
> First of all, Happy New Year to everyone in histoland.
>
> I am looking for companies that can sell all the equipment (refurbished)
> needed for a start up lab. I would like a package deal, everything
> included, IE Processor, embedding center, microtome, coverslipper, &
> waterbath all together.
>
> Vendors are welcome to respond.
> Thank you.
>
> Marsha Price
>
>
>
>
> ------------------------------
>
> Message: 18
> Date: Fri, 6 Jan 2006 08:57:38 -0800 (PST)
> From: Rene J Buesa
> Subject: Re: [Histonet] Processing/embedding question - your opinion
> To: foley1 , histonet@lists.utsouthwestern.edu
> Message-ID: <20060106165738.73387.qmail@web61219.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi Foley:
> I have seen that done in several places. I have also seen all the
> cassettes wrapped in aluminum foil and kept that way (like a pizza
> left-over).
> I cannot tell you what would be the consequences for the morphology or
> future tests.
> Theoretically speaking probably this practice will not affect because the
> tissue itself and all its components are supposedly embedded in paraffin
> that will just solidify.
> I personally do not like this to be done. For me, personally, it
> indicates laziness, indolence and even "disrespect" for the tissue sample.
> Once I was confronted with the need of keeping processed cassettes in a
> secure way before casting the blocks. My solution was to put all the
> cassettes in a shallow
> plastic container, place all the blocks in it, add melted paraffin and
> prepare one single block, as large as the container. When I was able to
> prepare the blocks individually, I melted the paraffin and prepared the
> blocks.
> Hope this will help you!
> René J.
>
> foley1 wrote:
> Does anyone routinely allow for the hot wax to drain off multiple
> cassettes
> of processed tissue and be held at room temperature for multiple days (6
> days) before embedding? What would the consequences be to morphology,
> possible immunohistochemistry and molecular (DNA) studies?
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ---------------------------------
> Yahoo! DSL Something to write home about. Just $16.99/mo. or less
>
> ------------------------------
>
> Message: 19
> Date: Fri, 6 Jan 2006 08:57:49 -0800
> From:
> Subject: [Histonet] Microwave slide drying vs. conventional slide
> drying
> To:
> Message-ID:
> <5B3B26C4B71D5E469892D1860ABE10EA7F1E7F@SHSEXVS01.int.samhealth.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> We are having a discussion about slide drying at out facility. How many of
> you out there use a microwave to dry your H&E slides before staining
> versus using a conventional 70 degree Celsius hot air slide drying oven
> for 20 minutes? I personally against microwave slide drying but the
> doctors like it because it takes less time (3 min. 30 sec. on medium
> setting). Thanks for your response.
>
> Lori A. Harris, HT (ASCP)
> Histology Section Leader
> GSRMC - Pathology
> 3600 NW Samaritan Drive
> Corvallis, OR 97330
> 1-541-768-6078
> lharris@samhealth.org
>
>
>
>
> Confidentiality Notice: This e-mail message, including any attachments, is
> for the sole use of the intended recipient(s) and may contain confidential
> and privileged information. Any unauthorized review, use, disclosure or
> distribution is prohibited. If you are not the intended recipient, please
> contact the sender by reply e-mail and destroy all copies of the original
> message.
>
>
> ------------------------------
>
> Message: 20
> Date: Fri, 6 Jan 2006 09:23:14 -0800 (PST)
> From: Steven Coakley
> Subject: [Histonet] sucrose liver
> To: Histonet@lists.utsouthwestern.edu
> Message-ID: <20060106172314.29262.qmail@web90204.mail.scd.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
>
> Good morning everyone, If anyone out there cryo-sections sucrose
> infiltrated fixed liver I'd like to know what temp there are sectioning at
> best. The mouse liver is fixed at 4C in formalin/PBS for 6 hours the
> 25-30% sucrose overnight also at 4C. I thought sucrose infiltrated tissue
> should section closer to -25C. Our chatter at anything above -18C.
>
> Steve
>
>
> ---------------------------------
> Yahoo! DSL Something to write home about. Just $16.99/mo. or less
>
> ------------------------------
>
> Message: 21
> Date: Fri, 6 Jan 2006 12:23:26 -0500
> From: "Hermina Borgerink"
> Subject: [Histonet] HTL certification
> To:
> Message-ID:
> <9AEEF1FB6254224AA355ED285F84916515A37AA7@EXCHVS2.medctr.ad.wfubmc.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Two of my techs will be taking the HTL certification this year. I
> checked the requirements on the ASCP Board of Registry home page and
> found that they needed experience in the following areas: fixation,
> processing, microtomy, and routine as well as special staining. I just
> wanted to double check with those of you who have recently taken their
> Board Certifications that EM and ISH experience are no longer required.
> When I did my HTL certification there still were questions pertaining to
> both topics, and I just want to make sure they won't be faced with
> questions they are not trained in. Any response would be greatly
> appreciated and I thank you in advance.
> Hermina
>
>
> Hermina M. Borgerink, BA, HT(ASCP)HTL, QIHC
> Wake Forest University Health Sciences
> Department of Pathology
> Medical Center Blvd.
> Winston-Salem, NC 27157
> Tel. (336) 716-1538
> Fax. (336) 716-1515
> e-mail hborgeri@wfubmc.edu
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 22
> Date: Fri, 6 Jan 2006 11:32:30 -0600
> From: "Bonnie Whitaker"
> Subject: RE: [Histonet] Processing/embedding question - your opinion
> To: "'Rene J Buesa'" ,
>
> Message-ID: <000001c612e7$2be04060$3601a8c0@brownpathology.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Rene,
>
> What is "lazy and indolent" about saving processed tissue without
> embedding
> it? I frequently collect many tissues for embedding in multi-tissue
> blocks,
> and I see no point in embedding the tissue temporarily while I continue to
> collect additional material. It makes no difference if the paraffin
> coating
> is minimal or a full block. You can also store a lot more control tissue
> in
> a small area if it is maintained in unembedded cassettes. One cassette
> can
> hold several blocks worth of material.
>
> Bonnie Whitaker
> Lab Manager
> Brown & Associates Medical Laboratories
> 8076 El Rio
> Houston, Texas 77054
> 713-741-6677
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J
> Buesa
> Sent: Friday, January 06, 2006 10:58 AM
> To: foley1; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Processing/embedding question - your opinion
>
>
> Hi Foley:
> I have seen that done in several places. I have also seen all the
> cassettes wrapped in aluminum foil and kept that way (like a pizza
> left-over).
> I cannot tell you what would be the consequences for the morphology or
> future tests.
> Theoretically speaking probably this practice will not affect because the
> tissue itself and all its components are supposedly embedded in paraffin
> that will just solidify.
> I personally do not like this to be done. For me, personally, it
> indicates
> laziness, indolence and even "disrespect" for the tissue sample.
> Once I was confronted with the need of keeping processed cassettes in a
> secure way before casting the blocks. My solution was to put all the
> cassettes in a shallow
> plastic container, place all the blocks in it, add melted paraffin and
> prepare one single block, as large as the container. When I was able to
> prepare the blocks individually, I melted the paraffin and prepared the
> blocks.
> Hope this will help you!
> René J.
>
> foley1 wrote:
> Does anyone routinely allow for the hot wax to drain off multiple
> cassettes of processed tissue and be held at room temperature for multiple
> days (6
> days) before embedding? What would the consequences be to morphology,
> possible immunohistochemistry and molecular (DNA) studies?
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ---------------------------------
> Yahoo! DSL Something to write home about. Just $16.99/mo. or less
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 23
> Date: Fri, 6 Jan 2006 09:38:07 -0800
> From: "Laurie Colbert"
> Subject: RE: [Histonet] Microwave slide drying vs. conventional slide
> drying
> To: , "Histonet (E-mail)"
>
> Message-ID:
> <0BE6ADFAE4E7E04496BF21ABD346628005653929@EXCHANGE1.huntingtonhospital.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Lori,
>
> We don't use either. We simply put our drained slides on the hot plate
> for about 5 minutes. In previous jobs, we always used an oven, and when I
> came here I was really nervous about just using the hot plate - but it
> works!
>
> Laurie Colbert
> Huntington Hospital
> Pasadena, CA
>
> -----Original Message-----
> From: lharris@samhealth.org [mailto:lharris@samhealth.org]
> Sent: Friday, January 06, 2006 8:58 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Microwave slide drying vs. conventional slide drying
>
>
> We are having a discussion about slide drying at out facility. How many of
> you out there use a microwave to dry your H&E slides before staining
> versus using a conventional 70 degree Celsius hot air slide drying oven
> for 20 minutes? I personally against microwave slide drying but the
> doctors like it because it takes less time (3 min. 30 sec. on medium
> setting). Thanks for your response.
>
> Lori A. Harris, HT (ASCP)
> Histology Section Leader
> GSRMC - Pathology
> 3600 NW Samaritan Drive
> Corvallis, OR 97330
> 1-541-768-6078
> lharris@samhealth.org
>
>
>
>
> Confidentiality Notice: This e-mail message, including any attachments, is
> for the sole use of the intended recipient(s) and may contain confidential
> and privileged information. Any unauthorized review, use, disclosure or
> distribution is prohibited. If you are not the intended recipient, please
> contact the sender by reply e-mail and destroy all copies of the original
> message.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 24
> Date: Fri, 6 Jan 2006 09:43:24 -0800 (PST)
> From: Rene J Buesa
> Subject: RE: [Histonet] Processing/embedding question - your opinion
> To: Bonnie Whitaker ,
> histonet@lists.utsouthwestern.edu
> Message-ID: <20060106174324.46935.qmail@web61222.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Bonnie:
> That is my personal and very particular opinion, and I think I am
> entitled to that,
> in the same way that you have expressed yours.
> I believe that this is covered by the freedom of expression, is it not?
> René J.
>
> Bonnie Whitaker wrote:
> Rene,
>
> What is "lazy and indolent" about saving processed tissue without
> embedding
> it? I frequently collect many tissues for embedding in multi-tissue
> blocks,
> and I see no point in embedding the tissue temporarily while I continue to
> collect additional material. It makes no difference if the paraffin
> coating
> is minimal or a full block. You can also store a lot more control tissue
> in
> a small area if it is maintained in unembedded cassettes. One cassette can
> hold several blocks worth of material.
>
> Bonnie Whitaker
> Lab Manager
> Brown & Associates Medical Laboratories
> 8076 El Rio
> Houston, Texas 77054
> 713-741-6677
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J
> Buesa
> Sent: Friday, January 06, 2006 10:58 AM
> To: foley1; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Processing/embedding question - your opinion
>
>
> Hi Foley:
> I have seen that done in several places. I have also seen all the
> cassettes wrapped in aluminum foil and kept that way (like a pizza
> left-over).
> I cannot tell you what would be the consequences for the morphology or
> future tests.
> Theoretically speaking probably this practice will not affect because the
> tissue itself and all its components are supposedly embedded in paraffin
> that will just solidify.
> I personally do not like this to be done. For me, personally, it indicates
> laziness, indolence and even "disrespect" for the tissue sample.
> Once I was confronted with the need of keeping processed cassettes in a
> secure way before casting the blocks. My solution was to put all the
> cassettes in a shallow
> plastic container, place all the blocks in it, add melted paraffin and
> prepare one single block, as large as the container. When I was able to
> prepare the blocks individually, I melted the paraffin and prepared the
> blocks.
> Hope this will help you!
> René J.
>
> foley1 wrote:
> Does anyone routinely allow for the hot wax to drain off multiple
> cassettes of processed tissue and be held at room temperature for multiple
> days (6
> days) before embedding? What would the consequences be to morphology,
> possible immunohistochemistry and molecular (DNA) studies?
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ---------------------------------
> Yahoo! DSL Something to write home about. Just $16.99/mo. or less
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>
> ---------------------------------
> Yahoo! DSL Something to write home about. Just $16.99/mo. or less
>
> ------------------------------
>
> Message: 25
> Date: Fri, 6 Jan 2006 11:51:03 -0600
> From: "Allen, Rhonda"
> Subject: RE: [Histonet] Processing/embedding question - your opinion
> To: "Rene J Buesa" , "Bonnie Whitaker"
> ,
> Message-ID:
>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Renee,
> It may be your opinion, but to those of us who have done it out of
> necessity, not being "lazy and indolent", it sounds like you are trying to
> make us feel "lazy and indolent".
>
> Rhonda Allen BA HT(ASCP)HTL, QIHC
> Histotechnology Specialist II
> Stowers Institute
> 1000 E. 50th Street
> Kansas City, MO 64110
> 816-926-4305
>
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J
> Buesa
> Sent: Friday, January 06, 2006 11:43 AM
> To: Bonnie Whitaker; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Processing/embedding question - your opinion
>
>
> Bonnie:
> That is my personal and very particular opinion, and I think I am
> entitled to that,
> in the same way that you have expressed yours.
> I believe that this is covered by the freedom of expression, is it not?
> René J.
>
> Bonnie Whitaker wrote:
> Rene,
>
> What is "lazy and indolent" about saving processed tissue without
> embedding it? I frequently collect many tissues for embedding in
> multi-tissue blocks, and I see no point in embedding the tissue
> temporarily while I continue to collect additional material. It makes no
> difference if the paraffin coating is minimal or a full block. You can
> also store a lot more control tissue in a small area if it is maintained
> in unembedded cassettes. One cassette can hold several blocks worth of
> material.
>
> Bonnie Whitaker
> Lab Manager
> Brown & Associates Medical Laboratories
> 8076 El Rio
> Houston, Texas 77054
> 713-741-6677
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J
> Buesa
> Sent: Friday, January 06, 2006 10:58 AM
> To: foley1; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Processing/embedding question - your opinion
>
>
> Hi Foley:
> I have seen that done in several places. I have also seen all the
> cassettes wrapped in aluminum foil and kept that way (like a pizza
> left-over). I cannot tell you what would be the consequences for the
> morphology or future tests. Theoretically speaking probably this practice
> will not affect because the tissue itself and all its components are
> supposedly embedded in paraffin that will just solidify. I personally do
> not like this to be done. For me, personally, it indicates laziness,
> indolence and even "disrespect" for the tissue sample. Once I was
> confronted with the need of keeping processed cassettes in a secure way
> before casting the blocks. My solution was to put all the cassettes in a
> shallow plastic container, place all the blocks in it, add melted paraffin
> and prepare one single block, as large as the container. When I was able
> to prepare the blocks individually, I melted the paraffin and prepared the
> blocks. Hope this will help you! René J.
>
> foley1 wrote:
> Does anyone routinely allow for the hot wax to drain off multiple
> cassettes of processed tissue and be held at room temperature for multiple
> days (6
> days) before embedding? What would the consequences be to morphology,
> possible immunohistochemistry and molecular (DNA) studies?
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ---------------------------------
> Yahoo! DSL Something to write home about. Just $16.99/mo. or less
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>
> ---------------------------------
> Yahoo! DSL Something to write home about. Just $16.99/mo. or less
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 26
> Date: Fri, 6 Jan 2006 11:53:08 -0600
> From: "Harrison, Sandra C."
> Subject: [Histonet] microwave tissue processing
> To:
> Message-ID:
> <736E8889E98B8F4FBBD29FDFEC8074BA49BF79@VHAV23MSGA2.v23.med.va.gov>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi,
>
> I'm looking into microwave tissue processors. If you are using one,
> please tell me the brand and whether you are pleased with the results.
> Do you find the instrument reliable?
>
> Thanks, Histonetters.
>
> Sandy
>
>
>
> ------------------------------
>
> Message: 27
> Date: Fri, 6 Jan 2006 09:54:58 -0800
> From: "Trajkovic, Dusko"
> Subject: RE: [Histonet] Processing/embedding question - your opinion
> To:
> Message-ID:
> <3AD0BD3142459B4E9B12CBEAFF2B89B2D896AB@lajamrexm01.amer.pfizer.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I have yet to see any published information stating that un-embedded
> tissue left to solidify, is damaging in any way or form. My colleagues an
> I in research, have done it numerous times, for various reasons, non were
> for being lazy.
>
> Dusko Trajkovic
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J
> Buesa
> Sent: Friday, January 06, 2006 9:43 AM
> To: Bonnie Whitaker; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Processing/embedding question - your opinion
>
> Bonnie:
> That is my personal and very particular opinion, and I think I am
> entitled to that,
> in the same way that you have expressed yours.
> I believe that this is covered by the freedom of expression, is it not?
> René J.
>
> Bonnie Whitaker wrote:
> Rene,
>
> What is "lazy and indolent" about saving processed tissue without
> embedding
> it? I frequently collect many tissues for embedding in multi-tissue
> blocks,
> and I see no point in embedding the tissue temporarily while I continue to
> collect additional material. It makes no difference if the paraffin
> coating
> is minimal or a full block. You can also store a lot more control tissue
> in
> a small area if it is maintained in unembedded cassettes. One cassette can
> hold several blocks worth of material.
>
> Bonnie Whitaker
> Lab Manager
> Brown & Associates Medical Laboratories
> 8076 El Rio
> Houston, Texas 77054
> 713-741-6677
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J
> Buesa
> Sent: Friday, January 06, 2006 10:58 AM
> To: foley1; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Processing/embedding question - your opinion
>
>
> Hi Foley:
> I have seen that done in several places. I have also seen all the
> cassettes wrapped in aluminum foil and kept that way (like a pizza
> left-over).
> I cannot tell you what would be the consequences for the morphology or
> future tests.
> Theoretically speaking probably this practice will not affect because the
> tissue itself and all its components are supposedly embedded in paraffin
> that will just solidify.
> I personally do not like this to be done. For me, personally, it indicates
> laziness, indolence and even "disrespect" for the tissue sample.
> Once I was confronted with the need of keeping processed cassettes in a
> secure way before casting the blocks. My solution was to put all the
> cassettes in a shallow
> plastic container, place all the blocks in it, add melted paraffin and
> prepare one single block, as large as the container. When I was able to
> prepare the blocks individually, I melted the paraffin and prepared the
> blocks.
> Hope this will help you!
> René J.
>
> foley1 wrote:
> Does anyone routinely allow for the hot wax to drain off multiple
> cassettes of processed tissue and be held at room temperature for multiple
> days (6
> days) before embedding? What would the consequences be to morphology,
> possible immunohistochemistry and molecular (DNA) studies?
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ---------------------------------
> Yahoo! DSL Something to write home about. Just $16.99/mo. or less
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>
> ---------------------------------
> Yahoo! DSL Something to write home about. Just $16.99/mo. or less
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> ----------------------------------------------------------------------
> LEGAL NOTICE
> Unless expressly stated otherwise, this message is confidential and may be
> privileged. It is intended for the addressee(s) only. Access to this
> E-mail by anyone else is unauthorized. If you are not an addressee, any
> disclosure or copying of the contents of this E-mail or any action taken
> (or not taken) in reliance on it is unauthorized and may be unlawful. If
> you are not an addressee, please inform the sender immediately.
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 26, Issue 4
> ***************************************
>
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