[Histonet] Plastic sectioning
Hi all
I am trying to cut 20-30um plastic sections of Zebrafish brain. The
fish was perfused with AFA (alcohol-formalin-acetic acid) for more
than a month and treated with Technovit 7100 for plastic sections.
Then the sections were stained with Thionin.
(Thionin 15m à running tap water 5m à rinse in dH2O à Dry à DePex) But
it got shattering and lots of knife marks on it. Any suggestion for
embedding and staining will be very appreciated.
Best,
Sunny
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>