[Histonet] Plastic sectioning

From:Sunhwa Lee

Hi all

I am trying to cut 20-30um plastic sections of Zebrafish brain.  The
fish was perfused with AFA (alcohol-formalin-acetic acid) for more
than a month and treated with Technovit 7100 for plastic sections.
Then the sections were stained with Thionin.
(Thionin 15m  running tap water 5m  rinse in dH2O  Dry  DePex) But
it got shattering and lots of knife marks on it.  Any suggestion for
embedding and staining will be very appreciated.


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