[Histonet] 50 micron mouse brain

From:"Atoska S. Gentry"

Hello, will someone please give me pointers on obtaining good 50u sections 
of PFA drop fixed and/or immersion fixed coronal paraffin sections of mouse 
brain? As I section they tend to roll up in tight rolls. I've tried 
sectioning at room temp. vs. chilled sections. Any assistance provided will 
be greatly appreciated. Thanks, Atoska


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