Re: [Histonet] endogenous alk phos
An older method of inhibiting endogenous alkaline
phosphatase is to treat the section with Lugol's or
Gram's iodine solution. Obviously not highly
specific, but does that matter for your purposes?
According to Humason's Animal Tissue Techniques,
alkaline phosphatase can also be inhibited by EDTA,
formaldehyde, KMnO4, cysteine or sodium arsenate.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
"Emerson, Rachael" wrote:
> Hello. Is there another method(s) to block endogenous alkaline phosphotase
> in mice, on frozen sections, besides levamisole?
> Rachael L. Emerson
> Center for Human Genetics and Molecular Pediatric Diseases
> University of Rochester Medical Center
> 575 Elmwood Avenue MRBX 1.11301
> Rochester, NY 14642
> Tel (585) 275-5073
> Fax (585) 276-0232
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