Re: [Histonet] cell characterization labels/stain
Thanks for your suggestion! I had a look at the website and spoke to their
US distributor, unfortunately, no-one has tested it in porcine tissue. I
still want to try to find out the epitopes to see if there is a possiblity
If I make any progress or I get any other responses I will definately let
Thanks again for the suggestion.
> Couldn't you just stain with an antibody that stains Fibroblasts?
> of antibodies for staining Fibroblasts in human, mouse and rat tissues... ... Antibodies to human Fibroblasts. ...
> www.acris-antibodies.com/focuson/focuson-new-08.html - 57k - Cached - Similar pages
> I'd be interested in hearing what responses you get. I'm working with endothelium cells.
> Senior Research Assistant
> BioMedical Engineering, OGI, OHSU.
> ----- Original Message -----
> From: Y. Wang
> To: Histonet@lists.utsouthwestern.edu
> Sent: Friday, January 28, 2005 3:55 PM
> Subject: [Histonet] cell characterization labels/stain
> I have a question about cell characterization. I know this isn't exactly
> your normal histology question but I've learnt so much from the people on
> this list in the past I was hoping someone would have a suggestion.
> We have started a primary cell culture from porcine anterior cruciate
> ligament (ACL). We have so far harvested the ACL, minced it and placed the
> pieces in culture to let the cells grow out of the pieces. After 5 days or
> so we have cell out growth from the pieces, however, we have cells with a
> variety of cell morphologies. I always thought we could generally tell
> cell type from the cell morphology however, it is proving to be rather
> difficult as we have a range of 'spindle-like' cells from very
> spindle-like to sort of spindle like but a bit more triangular.
> So, my question is: Does anyone know of a label/stain that can be used to
> characterize (ideally ACL) fibroblasts so that we can prove that our cells
> are ligament fibroblasts and not something else.
> I have done a search in some cell culture books, on the web and of past
> papers. I've even found images of what people think are ligament
> fibroblasts growing out of ligament pieces (ACL and MCL) however, I can't
> seem to find any information on characterizing these cells with something
> more reliable than saying 'these are fibroblasts because they have the
> characteristic spindle-like morphology'(am I not looking in the right
> places or with the correct key words?). The closest I've come was a paper
> on primary culture of urethra fibroblasts, which they identified as being
> fibroblasts because they were not smooth muscle cells (labeled for SM
> actin) or endothelial cells (labeled with an endothelial cell marker)!
> Any suggestions would be greatly appreciated.
> Yak-Nam Wang
> Senior Fellow
> Department of Bioengineering
> University of Washington
> Box 357962
> Seattle, WA 98195
> Tel.: (206)-221-5873
> Fax.: (206)-221-5874
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