Re: [Histonet] bubbles

From:"Robyn Vazquez"

Did you make the glycerol fresh or make it and let it set over night? 
I do immunofluorescence and use a 1:10 glycerol/pbs solution.  Could you
use the same?

>>> Danielle Jin  1/24/2005 2:05:21 PM >>>


I am new to histonet. I hope someone out there can help me solve my
problem.   When I do muscle staining (eg. acid phosphatase), the last
step is use glycerol Gelatin to cover the coverslip.  Somehow it
trap some bubbles in the tissue section.  How can I get rid of the
bubbles?  Thanks

Danielle Jin

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