Re: [Histonet] bubbles
Danielle,
Did you make the glycerol fresh or make it and let it set over night?
I do immunofluorescence and use a 1:10 glycerol/pbs solution. Could you
use the same?
Robyn
OHSU
>>> Danielle Jin 1/24/2005 2:05:21 PM >>>
Hello,
I am new to histonet. I hope someone out there can help me solve my
problem. When I do muscle staining (eg. acid phosphatase), the last
step is use glycerol Gelatin to cover the coverslip. Somehow it
always
trap some bubbles in the tissue section. How can I get rid of the
bubbles? Thanks
Danielle Jin
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