Re: [Histonet] bubbles

From:"Janice A Mahoney"

First be sure there are no bubbles in the glycerol/gelatin.  Then be sure that the sliide is still wet (with distilled water) when you  coverslip.
Hope this helps.

>>> Danielle Jin  01/24/2005 4:05:21 PM >>>

I am new to histonet. I hope someone out there can help me solve my
problem.   When I do muscle staining (eg. acid phosphatase), the last
step is use glycerol Gelatin to cover the coverslip.  Somehow it always
trap some bubbles in the tissue section.  How can I get rid of the
bubbles?  Thanks

Danielle Jin

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