Re: [Histonet] aqueous mounting medium

From:John Kiernan

The product of the indigogenic reaction for 
beta-galactosidase is a pigment. It doesn't
dissolve in the solvents used for microscopy 
and should be OK in any mounting medium, 
resinous or aqueous. I've used 3 or 4 different
resinous media with products of indigogenic
reactions, and all have been OK. 

Amanda MacFarlane wrote:
> I have stained whole tissues for beta-galactosidase (X-gal) and am now
> cryosectioning the tissues. I have been fixing the sections in 4% PFA
> overnight at 4 degrees Celsius and cover slipping with 70% glycerol.
> Coverslipping with glycerol is a time-consuming pain in the butt so I've
> checked out glycerol vinyl alcohol aqueous (GVA) mounting solution (Zymed)
> and Supermount aqueous mounting medium (Biogenex) as alternatives. It
> appears that these products have not been tested with beta-gal staining,
> so I'm wondering if people use these products and/or  what people
> recommend. Thanks.
> Amanda MacFarlane, PhD
> Division of Nutritional Sciences
> Cornell University
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