Re: [Histonet] Use of Sta-On for Immuno stains

From:Gayle Callis

We bite the bullet, and buy Plus charge slides for ALL tissues and 
use  distilled water in flotation bath.  OR Polysine (Erie Scientific) - 
the poly l lysine commercial editions which allow one to manipulate 
sections a tidge onto slide surface.

Maybe a tidge more expense but with shopping around for a good discount, 
vendors are usually very good about this, it eliminates a lot of 
frustration and recuts.

Never looked back and everyone is happy.

At 08:10 AM 1/21/2005, you wrote:
>I was just wondering how other labs are handling this situation:  For
>prostate and breast core biopsies we cut extra slides for potential immuno 
>between the levels.  The problem is the use of adhesive in the water bath.
>Since we are cutting H&E's, there is the possibility of fall off during 
>if we DON'T use sta-on.  But using it increases the possibility of fall off
>during immunos staining.  And if we don't use the sta-on, we run the risk of
>fall off on ALL the cases the techs are cutting that day.
>I know there are lots of labs out there performing immunos, so I'd like to
>know how you've resolved this.

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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