Re: [Histonet] RE: Fluorescence staining

From:Gayle Callis

Chris and Eva,

Vectashield also come in a Hard Set, so you get permanent coverslipping (no 
coverglass sliding around!) as does the Molecular Probes Prolong Gold ready 
to use.

If you want a counterfluorescence marker for nuclei, and have the 
appropriate DAPI filter on microscope, you can buy these with DAPI which 
"counterstains via fluorescence" the DNA in cells.

At 12:52 AM 1/26/2005, you wrote:

>    Dear Eva,
>    To my knowledge most of the traditional fluorochromes like FITC,
>    rhodamine, doesn't stand dehydration at all. So in your case it fully
>    depends on what type of fluorochrome you end up with after the TSA
>    procedure.
>    If you end up with FITC, the use of VectaShield
>    ([1] for mounting is highly recommended for
>    the prevention of photobleaching.
>    Chris van der Loos, PhD
>    Dept. of Pathology
>    Academical Medical Center M2-230
>    Meibergdreef 9
>    NL-1105 AZ Amsterdam
>    The Netherlands
>    phone:  +31 20 5665631
>    fax:    +31 20 6960389
>    e-mail: [2]

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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