Re: [Histonet] PLP fixative
In a message dated 1/19/2005 5:12:42 PM US Mountain Standard Time,
> does anyone have the recipe for PLP fixative and how it is used?
> After fixation can the tissue (brain) be processed thru paraffin as usual?
> Cindi Roberts
> Neurology Research
> Henry Ford Hospital
The recipe for PLP (Periodate-Lysine-Paraformaldehyde) fixative and a sample
protocol (at least for immuno) using perfusion fixation is on the Chemicon
There are a few instances of using PLP with paraffin embedding, so the
processing should work OK. Whether you can retrieve reactivity from paraffin
sections seems to be related to the antigen. For example, see the NIEHS website:
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