Re: [Histonet] GLP lab staining question

From:Bryan Llewellyn

The way I was taught was to cut a series of sections, 50 or so, then stain 
the first and the last to make sure they were positive  If they both are, it 
is presumed that all the others are also.  If not, stain intermediate 
sections until one is positive, then use from the first one to that.

Bryan Llewellyn

----- Original Message ----- 
From: "pam plumlee" 
To: "HistoNet Server" 
Sent: Thursday, January 20, 2005 2:13 PM
Subject: [Histonet] GLP lab staining question

> Hello fellow histonetters:  I'm setting up a special
> stain control/slide bank for our research pharm lab.
> I'm curious how special stain controls are validated.
> Is it as simple as screening the stained slides/blocks
> as they are cut and determining if they are positive
> or not, along with the proper documentation?  Anyone
> care to share info?  Thanks, PP
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