Re: [Histonet] Ache staining querry

From:John Kiernan

Assuming "Ache staining" is acetylcholinesterase activity
histochemistry, not a painful bruise:

The surface of the specimen will have been fixed more than 
the inside. AChE activity resists short fixation
in formaldehyde (such as overnight). Perhaps the outside
of the block has had more than is good for the enzyme.

In small specimens of animal tissues, both the choline 
esterases (and other esterases) are well preserved by 
overnight fixation in formaldehyde.
Human brains need much longer because of the large size,
and this can be expected to wreck enzyme activities. You
might still be able to stain the AChE protein with an 
immunohistochemical method.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
Arvind Pundir wrote:
> i do Ache  staining frequently on human brain sections, but  facing problem that there is no staining at the edges of my section can anyone sort what it may be due to
> thanks in advance for any suggestion or advice
> Arvind Singh Pundir
> National Brain Research Centre
>   ------------------------------------------------------------
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