RE: [Histonet] Use of Sta-On for Immuno stains

From:Shirley Powell

In the lab where I do PRN work, they sit a small glass dish of deionized
water inside one end of regular waterbath where they use Sta-On. The
deionized water gets heated with the regular waterbath and immuno sections
can be floated on the deionized  water and picked up with positive charged
slides.  If your waterbath is not large enough to accommodate a small dish
in it, set up a second waterbath beside it.


-----Original Message-----
[]On Behalf Of
Sent: Friday, January 21, 2005 10:11 AM
Subject: [Histonet] Use of Sta-On for Immuno stains

I was just wondering how other labs are handling this situation:  For
prostate and breast core biopsies we cut extra slides for potential immuno
between the levels.  The problem is the use of adhesive in the water bath.
Since we are cutting H&E's, there is the possibility of fall off during
if we DON'T use sta-on.  But using it increases the possibility of fall off
during immunos staining.  And if we don't use the sta-on, we run the risk of
fall off on ALL the cases the techs are cutting that day.

I know there are lots of labs out there performing immunos, so I'd like to
know how you've resolved this.
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