Have done some mice with a glut/formaldehyde mix. Do not know the
percentages off the top of my head. Following the perfusion we grossly
section the brain either coronally or sagittally usually to a 2-4 mm
thickness and them do a punch in the area of interest for EM. The remaining
specimen is post fixed for light microscopy in NBF for minimum of 24 hours
up to 5 days. I believe the EM crew post fixes there specimens as well. Nice
thing is that EM seems to be happy and we know exactly where the specimen
was taken because the punch is visible on the light microscopy slide. 

c

-----Original Message-----
From: Kristen Reynolds [mailto:kristenhinkle@yahoo.com] 
Sent: Wednesday, January 19, 2005 3:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Perfusion suggestions

I need advice on perfusion solutions for rat/monkey brains.  I need to use
the tissue for both light microscopy and EM.  Any suggestions?

Kristen Reynolds
UT Austin

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