Hal

When we have done vascular permeability studies in the past we have used
Evans Blue Dye (30 mg/kg in a volume of 4 ml/kg (mice) or 1 ml/kg
(rat)), injected IV when we are using capsaicin in a systemic model or
Evans blue dye (1 mg/kg in a volume of 1 ml/kg (rat)) is injected IV and
then 20 µl intradermal injections of test articles. I hope this helps. =20

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hawkins,
Hal K.
Sent: Tuesday, January 18, 2005 12:30 PM
To: Histonet
Subject: [Histonet] Monastral blue

 
We need to study changes in vascular permeability in animal models using
Monastral Blue B dye.  Apparently Sigma-Aldrich no longer supplies this
pigment.  Does anyone know of another source, or would anyone be willing
to share/sell us some?  Alternatively, is there another colloid that is
useful in the same way at the level of both light and electron
microscopy?  
 
Thanks,
Hal Hawkins
University of Texas Medical Branch
Galveston, Texas
hhawkins@utmb.edu
 
 
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