RE: [Histonet] ISH control probe

From:"Elizabeth Chlipala"

Patti
 
I think I can help you, but I need to know what your target is.
Typically we normally are going after the mRNA rather than the DNA that
has only one to a few copies and is duplexed.  So you would be more
concerned about the integrity of RNA rather than DNA.  If you are
looking for RNA then we use a probe that hits 18s rRNA that hits all
eucarya.  It's an oligo probe that you can get any oligo company to make
up with a fluor or hapten attached to it.  Its 5'GGGCATCACAGACCTG3' with
Tm = 52C.  
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti
Loykasek
Sent: Friday, January 21, 2005 12:14 PM
To: histonet
Subject: [Histonet] ISH control probe
 
I have a question for those folks doing ISH. What type of probe are you
using to determine that the DNA in the tissue is available for
hybridization? Thanks for the help.
 
Patti Loykasek
PhenoPath Laboratories
Seattle, WA
 
 
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