Is there a sure fire way of iliminating endogenous peroxidase from
granulecytes in acetone fixed cytospins for hrp IHC?  Can I solve this
problem without changing to a non-hrp detection enzyme?  I am trying to
stain T lymphocytes in cytospins of sputum.  I do get cd3 positive
lymphocyte looking cells, but agressive efforts has not completely blocked
endogenous peroxidase in some of the larger cells with granules.
Thanks for your help,
Patsy
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