Dear Ze Lu
   thank you very much to all of you replyed to my question.
   Subrata.
   --
   http://www.histosearch.com/homepages/DrSubrataKumarBiswas.html

   Message: 1
   Date: Fri, 21 Jan 2005 13:21:21 -0500
   From: "Ze Lu" 
   Subject: [Histonet] Re: NIH image J surface area unit
   To: 
   Message-ID: <00b801c4ffe6$02186290$1302a8c0@OPTIMUM2>
   Content-Type: text/plain; charset="iso-8859-1"
   Dear Subrata,
   It  needs  to do spatial calibration if you want to measure the actual
   unit. There is function called "set scale". First you should observe a
   standard  scale with known distance under microscope. Then measure the
   pixel  number  of  a  line  with  known  distance.  Using  "set scale"
   function, input actual length, unit, choose "global" option.
   There  is  also  a  plug  in  that  you  can  download.  It  is called
   "microscope  scale". So you don't need to calibrate everytime when you
   start the program.
   Ze Lu, Ph.D.
   Senior Scientist
   Optimum Therapeutics
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