[Histonet] Re: NIH image J surface area unit
It needs to do spatial calibration if you want to measure the actual unit. There is function called "set scale". First you should observe a standard scale with known distance under microscope. Then measure the pixel number of a line with known distance. Using "set scale" function, input actual length, unit, choose "global" option.
There is also a plug in that you can download. It is called "microscope scale". So you don't need to calibrate everytime when you start the program.
Ze Lu, Ph.D.
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