[Histonet] Re: NIH image J surface area unit

From:"Ze Lu"

Dear Subrata,


It needs to do spatial calibration if you want to measure the actual unit. There is function called "set scale". First you should observe a standard scale with known distance under microscope. Then measure the pixel number of a line with known distance. Using "set scale" function, input actual length, unit, choose "global" option. 


There is also a plug in that you can download. It is called "microscope scale". So you don't need to calibrate everytime when you start the program.


Ze Lu, Ph.D.

Senior Scientist

Optimum Therapeutics
Histonet mailing list

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