[Histonet] Re: Has anyone tried decalifying bone for frozen sections without fixing first?
It has been done and is published. I have not done it.
1) Jonsson R et al. A demineralization procedure for
immunohistopathological use: EDTA treatment preserves lymphoid cell
surface antigens J Immunological Methods 88:109114,1986.
This group had very good staining after a LONG EDTA decalcification on
murine hindlegs (14 days) , a rather tedious protocol but their CD markers
were well stained and tissue was preserved. After snap freezing EDTA
decalcified bone, they cut frozen sections, and fixed them with acetone,
did IHC for T and B lymphocytes, Ia antigens.
2) Kim L. Kusser and Troy D. Randall
Simultaneous Detection of EGFP and Cell Surface Markers by Fluorescence
Microscopy in Lymphoid Tissues
J. Histochem. Cytochem., Jan 2003; 51: 5 - 14.
Kusser also cites other publications in her article that may be helpful.
At 09:02 AM 1/28/2005, you wrote:
>Has anyone tried decalifying bone for frozen sections without fixing
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