Hi Patty,

This was discussed  on Histonet just a couple of months ago, in 
detail.  You have to remove the plastic with 60C xylene so there is NO 
plastic left in the tissue.  Neil Hand did this nicely, and did at least 3 
changes to get the MMA out, then rehydrate section and stain - you may have 
to adjust for your H&E protocol, whatever tweaks are necessary.   One nice 
lady put her staining protocol on Histonet.


Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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