[Histonet] RE: endogenous peroxidase blues
Before everybody is trying to solve their problem of endogenous
peroxidase activity with Dual Enzyme Blocking Reagent as Veronique
wrote us, I would like to make a comment. This stuff works great
indeed in many cases, that's true. But I also found some antigens
that didn't survive this treatement at all. So if you are going to
test Dual Enzyme Blocking Reagent, keep this in mind and be sure to
have the right controls involved.
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center M2-230
NL-1105 AZ Amsterdam
phone: +31 20 5665631
fax: +31 20 6960389
----- Original Message -----
From "Veronique Andriessen"
Date Fri, 28 Jan 2005 11:50:40 +0100
To "Histonet" , "Patsy Ruegg"
Subject RE: [Histonet] endogenous peroxidase blues
I have encountered somewhat of the same problem with endogenous
in acetone fixed frozen sections of acute pancreatitis, which is
with white blood cells. I tested several ways of blocking, home made
commercial including glucose oxidase. I never got it to work properly
(believe me, I've tried!). What worked for me in the end was a new
by DAKO called "Dual Endogenous Enzyme Blocking Reagent" (S2003)
Mr. van der Loos!) It should work on cell preparations, frozen tissue
FFPE tissue (according to the manual). I only have tested it on frozen
Just 10 minutes incubation at RT.
This is just my honest opinion, I hope this can help you.
Veronique Andriessen BAS
Lab. Molecular Liver Cell Biology
Free University Brussels (VUB), Belgium
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