[Histonet] RE: Fluorescence staining
To my knowledge most of the traditional fluorochromes like FITC,
rhodamine, doesn't stand dehydration at all. So in your case it fully
depends on what type of fluorochrome you end up with after the TSA
If you end up with FITC, the use of VectaShield
(www.vectorlabs.com) for mounting is highly recommended for
the prevention of photobleaching.
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center M2-230
NL-1105 AZ Amsterdam
phone: +31 20 5665631
fax: +31 20 6960389
----- Original Message -----
Eva C Anderson
Tue, 25 Jan 2005 11:10:41 -0500
[Histonet] Fluorescence staining
We are looking into trying some Fluorescence staining. We want to try
a TSA Fluorescence System from PerkinElmer. The IHC protocol does
however leave me with some questions.
We always dehydrate our samples after staining. The protocol however
doesn't mention this. It only says that the samples can be
counterstained and mounted after incubation with the Fluorophore
Tyramide. Should we dehydrate and is there a particular mounting media
we should use to prolong the time that fluorescence can be detected?
Any help would be greatly appreciated.
Thanks in advance for all your help,
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