[Histonet] In situ pcr on paraffin rat brain sections

From:Lucy Teves

Our lab is interested in doing In Situ PCR on paraffin rat brain sections on
slides.  We are trying to identify location of an  increase or decrease of
assorted proteins.  We have arranged for a demo on the Eppendorf Mastercyler
which comes with a slide plate.

Can any one give me a basic protocol that works so I can test this machine.

Thanks in advance


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