From:"Timothy R. Wheelock"

Hi Sue:

I use Contrad 70, a liquid detergent, which you can find at Fisher 
Scientific on-line, or the Fisher catalogue. I dilute it down to 10%, 
 and  then immerse my coplin jars and flasks in it (in my case, for the 
Bielschowsky silver stain), either over the working day or overnight. I 
change it every 4 staining runs, just as a rule of thumb. You can 
probably get away with a less than 10% dilution,  shorter immersion 
times, and other changing schedules.

It is far safer than the concentrated Nitric Acid that I had been using 
for years. I had  an  accident with the Nitric Acid a while ago (major 
spill), which prompted me to find a safer substitute. Someone on 
Histonet suggested Contrad 70. It has worked just as well as pure Nitric 
Acid for my silver stains. They say that you can even dump it down the 
drain after use, but I always put it in waste container anyway. Check 
with other people on Histonet though, for their opinions about this product.

Tim Wheelock
Harvard Brain Tissue Resource Center
McLean Hospital
Belmont, MA

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