Re: [Histonet] oil red in MMA

From:Gayle Callis

I doubt this would work as the solvents (alcohols, clearants, monomers are 
really extracting the lipid, an important factor for making MMA work and 
polymerize anyway is to NOT have fat in bone.

The dye would not penetrate the plastic anyway, you would have to remove 
the plastic completely and that step would continue to remove the fat from 
your tissue plus your sections are very thick.

If you had post fixed your tissue with osmium tetroxide before processing, 
you may have had more success in detecting/retaining lipids.

, At 05:42 AM 1/7/2005, you wrote:
>Hi dear histonnetters and happy new year 2005 !!
>does anyone have a protocol of oil red staining in tissu embedded in MMA 
>(mixture of butylmetacrylate and methylmetacrylate)?
>i would like to stain fats in soft tissu on titane. My sections are 50Um 
>thikness.
>thank you in advance
>Myriam
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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