Re: [Histonet] oil red in MMA
I doubt this would work as the solvents (alcohols, clearants, monomers are
really extracting the lipid, an important factor for making MMA work and
polymerize anyway is to NOT have fat in bone.
The dye would not penetrate the plastic anyway, you would have to remove
the plastic completely and that step would continue to remove the fat from
your tissue plus your sections are very thick.
If you had post fixed your tissue with osmium tetroxide before processing,
you may have had more success in detecting/retaining lipids.
, At 05:42 AM 1/7/2005, you wrote:
>Hi dear histonnetters and happy new year 2005 !!
>does anyone have a protocol of oil red staining in tissu embedded in MMA
>(mixture of butylmetacrylate and methylmetacrylate)?
>i would like to stain fats in soft tissu on titane. My sections are 50Um
>thank you in advance
>Histonet mailing list
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Veterinary Molecular Biology
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