Re: [Histonet] endothelial cell marker other than PECAM
I haven't started yet , but am about to try von Willebrand's factor (
Factor VIII ). I'll be doing it on rat, first in FFPE to see if it works,
and then on osmium fixed Epon embedded. Wish me luck!
I chose Factor 8 because of a paper by Ulger et al , Anat
Histol Embryol. 2002 Feb:31(1):31-5 where he compares various
rat endothelial cell markers : Factor 8 , RECA-1, PECAM-1 , ICAM-
1, OX-43 and ZO-1.
I am buying my anti body from Biocare Medical and their tech
support suggests trying a pepsin treatment as they use it on the
FFPE tissue at 37 C for 5 min.
Hope this helps , don't hesitate to email me if you have
any more questions,
Margaret Horne ,
Histology Teaching Assistant,
Dept. of B.SC.,
Atlantic Veterinary College, U.P.E.I.,
550 University Ave., Charlottetown,
P.E.I., C1A 4P3
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