Re: [Histonet] endothelial cell marker other than PECAM

From:"Margaret Horne"

Hi , 
   I haven't started yet , but am about to try von Willebrand's factor ( 
Factor VIII  ).   I'll be doing it on rat,  first in FFPE to see if it works, 
and then on osmium fixed Epon embedded.   Wish me luck!

              I chose Factor 8 because of a paper by Ulger et al , Anat 
Histol Embryol. 2002 Feb:31(1):31-5   where he compares various 
rat endothelial cell markers : Factor 8 , RECA-1, PECAM-1 , ICAM-
1, OX-43 and ZO-1.   

I am buying my anti body from  Biocare Medical and their tech 
support suggests trying a pepsin treatment as they use it on the 
FFPE tissue at 37 C for 5 min.  

               Hope this helps , don't hesitate to email me if you have 
any more questions, 

Margaret Horne ,
Histology Teaching Assistant,
Dept. of B.SC.,
Atlantic Veterinary College, U.P.E.I.,
550 University Ave., Charlottetown,
P.E.I.,  C1A 4P3 

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