Re: [Histonet] The "Stuff" to Find Lymph Nodes
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----- Original Message -----
From: "Patrick Paulusse"
To: "Histonet" ; "Smith Wanda"
Sent: Tuesday, January 04, 2005 6:58 PM
Subject: Re: [Histonet] The "Stuff" to Find Lymph Nodes
> Although I have not been fortunate enough to have tried some of the
> commercially available lymph node retrieval solutions, I do use a homemade
> solution which was outlined in an article (referenced below) that you
> be interested in trying. The recipe is as follows:
> 500 ml of absolute ethanol
> 170 ml distilled water
> 80 ml of 37% formaldehyde
> 50 ml of acetic acid
> According to the article the reagents must be added in this order (does
> anyone know why?). I've used this since 2001 with great success. I
> off the pericolic adipose tissue and leave it sit in the solution for 24
> hours and the smallest of lymph nodes show up white against the
> adipose tissue. I've also let it sit over the weekend and have seen no
> deleterious affects because of the prolonged treatment. If you try it let
> me know what you think.
> Article Reference:
> "GEWF solution: an inexpensive, simple, and effectiveaid for the retrieval
> of lymph nodes from colorectal cancer specimens". Ken J. Newell, Barry W.
> Sawka, Brian F. Rudick, David K. Driman. Arch Pathol Lab Medicine, May
> Patrick Paulusse
> Histology Supervisor
> Pembroke Regional Hospital
> Ontario, Canada
> ----- Original Message -----
> From: "Smith Wanda"
> Sent: Tuesday, January 04, 2005 3:40 PM
> Subject: [Histonet] The "Stuff" to Find Lymph Nodes
>> Dear Histonetters,
>> My mind has gone blank...and I need help please!
>> What are some of the commercial solutions available to help ID lymph
>> at the grossing table? What are your experiences, pro and con, of these
>> One of my Pathologist has read a few articles concerning finding lymph
>> on colon cancer cases. A high number of lymph nodes must be found, down
>> the 1-2 mm size nodes and we are interested in other's experiences.
>> Thanks for any and all info!!!
>> > Wanda G. Smith, HTL/HT(ASCP)
>> > Pathology Supervisor
>> > Trident Medical Center Laboratory Services
>> > *9330 Medical Plaza Drive, Charleston, SC 29406
>> > *843-797-4586 *fax 843-797-4296
>> > *firstname.lastname@example.org
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