Re: [Histonet] Sections floating off of slides

From:"Robyn Vazquez"

Would this work with frozen nasal bone?  

>>> Paul Bradbury  1/10/2005 7:45:46 PM >>>

Hi Becky,

Keeping brain sections on the slide has always been a problem. Over the

years, I have tried plain slides, coated slides, textured slides, etc.

Sometimes they seem to work, sometimes they fail, there does not seem
be a guaranteed method.

One adhesive that was very successful is methyl cellulose. A small 
amount sprinkled on the suface of the water bath and allowed to
was probably the best brain adhesive I have tried. Methyl cellulose is

available in most paint/decorating stores as an adhesive for 
wallpaper.   In low concentrations, it does not seem to react with most

stains and dyes, so the background stays clear. I have no idea how it 
reacts with immunohistochemical techniques, I have not used this stuff

since IHC became routine. Give it a try, it might work for you too.
up the sections onto the slides, allow them to drain and dry them for
hour or two at 60 degrees.

Paul Bradbury
Kamloops, BC

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