Re: [Histonet] Sections floating off of slides
Keeping brain sections on the slide has always been a problem. Over the
years, I have tried plain slides, coated slides, textured slides, etc.
Sometimes they seem to work, sometimes they fail, there does not seem to
be a guaranteed method.
One adhesive that was very successful is methyl cellulose. A small
amount sprinkled on the suface of the water bath and allowed to dissolve
was probably the best brain adhesive I have tried. Methyl cellulose is
available in most paint/decorating stores as an adhesive for
wallpaper. In low concentrations, it does not seem to react with most
stains and dyes, so the background stays clear. I have no idea how it
reacts with immunohistochemical techniques, I have not used this stuff
since IHC became routine. Give it a try, it might work for you too. Pick
up the sections onto the slides, allow them to drain and dry them for an
hour or two at 60 degrees.
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